Harvard College

The Governors of the University of Alberta

Massachusetts Institute of Technology

Daniel Hochbaum

Adam Ezra Cohen

Robert Earl Campbell

Joel Kralj

Daniel Jed Harrison

Adam D Douglass

Peng Zou

Samouil Leon Farhi

Yongxin Zhao

Edward Stuart Boyden

Brian Yichiun Chow

Nathan Klapoetke

Yongku Peter Cho

Gane K S Wong

Provided herein are variants of an archaerhodopsin useful for application such as optical measurement of membrane potential. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, cells comprising the polynucleotides, and cells comprising the polypeptides; and methods of using the variants.

Optogenetic probes for measuring membrane potential

The invention, in some aspects relates to light-activated ion channel polypeptides and encoding nucleic acids and also relates in part to compositions comprising light-activated ion channel polypeptides and methods using light-activated ion channel polypeptides to alter cell activity and function.

Blue-light-activated ion channel polypeptides and uses thereof

The invention provides methods for characterizing cellular physiology by incorporating into an electrically excitable cell an optical reporter of, and an optical actuator of, electrical activity. A signal is obtained from the optical reporter in response to a stimulation of the cell. Either or both of the optical reporter and actuator may be based on genetically-encoded rhodopsins incorporated into the cell. The invention provides all optical methods that may be used instead of, or as a complement to, traditional patch clamp technologies and that can provide rapid, accurate, and flexible assays of cellular physiology.

Systems, methods, and workflows for optogenetics analysis

Growing community of inventors

Cambridge, MA, United States of America

Daniel Hochbaum