Bioceres S.a.

Consejo Nacional De Investigaciones Cientificas Y Tecnicas (conicet)

Universidad Nacional Del Litoral

Other

Daniel Hector Gonzalez

Raquel Lia Chan

Carlos Alberto Dezar

Gabriela Marisa Gago

Lia Raquel Chan

Julieta Virginia Cabello

Eva C Rueda

Graciela C Curi

The invention relates to polynucleotides encoding a modified HaHB4 transcription factor and polynucleotides encoding functionally active fragments and variants of a modified HaHB4 transcription factor as well as vectors and host cells containing these polynucleotides and the polypeptides encoded by these polynucleotides. The invention also encompasses transgenic host cells, plants, seed, pollen, and plant parts containing the polypeptides and/or polynucleotides of the invention. The invention further encompasses methods of producing transgenic host cells, plants, seed, pollen, and plant parts and the processed plant products produced from these transgenic hosts.

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A new transcription factor coding gene induced by water deficit or abscisic acid of, having a homeodomain associated to a leucine zipper, was characterized. The transcription factor is useful to be cloned in DNA constructions for transforming host cells and plants. The transgenic plants comprising the transcription factor gene are tolerant and resistant to harmful environmental conditions such as water stress and high salinity. A nucleic acid promoting sequence is also provided wherein the sequence is induced by water deficit or abscisic acid. Constructions, host cells and transgenic plants that comprise the transcription factor gene are provided.

The present invention refers to a gene fromencoding a transcription factor that comprises a homeodomain associated with a leucine zipper. This gene is named Hahb-10. The transcription factor Hahb-10 can be used in DNA constructs to transform host cells and plants. Transgenic plants that overexpress this transcription factor are more tolerant to herbicides, and have a shorter life cycle.

An isolated DNA molecule for enhancing gene expression of a coding sequence, fragment, genetic variant, cassette, vector, cell, plant and seed containing said molecule, wherein the molecule comprises introns for induce an increase in the expression level of a transgene, useful in DNA constructions for transforming plant cells, wherein the cell or plant has a construction containing one of these introns under control of a promoter and upstream of a coding sequence stably integrated in its genome displaying higher expression levels as compared to non-transformed cells or plants, or cells or plants transformed with constructions that lack the inventive intron, wherein the sequences of 5'-non-coding sequences of the genes mentioned above also comprise promoters and exonic sequences in DNA constructions displaying synergism with the effect produced by the intron, and wherein the 5&#x2032;-non-coding sequences of COX5-c genes lacking said intron promote tissue-specific expression in pollen when they are comprised in DNA constructions for plant transformation.

Growing community of inventors

Santa Fe, Argentina

Daniel Hector Gonzalez