Buhlmann Laboratories Ag

Ciba-geigy Corporation

Forschungszentrum Juelich Gmbh

Bioversys Ag

Daniel Gygax

Jakob Weber

Oreste Ghisalba

Thomas Jermann

Lara Hasan

Michel-Angelo Sciotti

Gottfried Sedelmeier

Christian Wandrey

Peter Schneider

Udo Kragl

Marc Gitzinger

Hans-Peter Schar

Gregor Naef

André Scholer

Marcel Tigges

Peter Spies

Michael Brand

Patrik Zueger

Birgit Schellhorn

Mark Sephton

Assaf Levi

Lukas Steiner

Cédric Wernli

Michel Pieren

Rainer Riedl

Elke Schmidt

Rente Lattmann

Jacob Weber

A hand-held applicator () for collecting, mixing, diluting and discharging a sample liquid, comprising a main body () having an inlet-aperture (), an outlet-aperture () and a chamber () for storing a liquid solution, wherein the chamber is connected to the inlet-aperture and the outlet-aperture, a lid () for covering the inlet-aperture and a tube (), moveably supported in the inlet-aperture when the lid is covering the upper portion of the main body, wherein the tube () is moveable between a predetermined position outside the chamber and a position, in which the tube is at least partly located in the chamber.

Hand-held applicator

The invention relates to compounds and pharmaceutical compositions useful in combination with tetracyclines in the treatment of bacterial infections caused by Gram-positive and Gram-negative pathogens, with particular efficacy in tetracycline resistant strains. These compounds specifically bind to TetR and therefore prevent the transcriptional activation of tet resistance genes. The compounds have a potentiating effect on the activity of members of the tetracycline family, in particular of tetracycline, minocycline, doxycycline and tigecycline, in the treatment of tetracycline susceptible, intermediate and tetracycline resistant pathogens.

Composition for treatment of pathogens that are resistant to tetracyclines

The invention relates to methods for the determination of vitamin B6 in samples as well as to reagent compositions for assaying a sample for vitamin B6 and to a test kit suitable for carrying out the methods according to the present invention. Further, the invention relates to the use of such methods for the application to different analyzing devices such as micro-titer plate readers and fully automated clinical chemistry analyzers (autoanalyzers).

Method for the determination of the concentration of vitamin B6 in a sample

Methods to determine the concentration of gamma-hydroxy butyric acid (GHB) in a sample as well as compositions and a kit suitable for carrying out said methods. Also, the use of the methods for application on a microtiter plate or an auto-analyzer.

Methods for determining the concentration of gamma-hydroxy butyric acid (GHB) in a sample

The invention relates to a preferably continuous process for the preparation of R- or S-2-hydroxy-4-phenylbutyric acid in very high enantiomeric purity, which comprises reducing 2-oxo-4-phenylbutyric acid with the enzyme D-lactate dehydrogenase from Staphylococcus epidermidis or with the enzyme L-lactate dehydrogenase from bovine heart, respectively, in the presence of NAD(H) and formate or ethanol and formate dehydrogenase or alcohol dehydrogenase, respectively. R-2-hydroxy-4-phenylbutyric acid is a valuable intermediate in the preparation of ACE inhibitors or their precursors.

Process for the preparation of hydroxy acids

A process for obtaining N-acetylneuraminic acid from N-acetylglucosamine is disclosed. The process is carried out in a reactor which contains both N-acylglucosamine-2-epimerase (E.C. 5.1.3.8) which isomerizes GlcNAc into ManNAc, and N-acetylneuraminic acid pyruvate lyase (E.C. 4.1.3.3) which catalyzes the reaction of the resulting ManNAc with pyruvic acid to give Neu5Ac. GlcNAc and Pyr are fed into the reactor and Neu5Ac is obtained in the outflow. The process is preferably carried out continuously and in particular in an enzyme membrane reactor at pH 7.5 and 25.degree. C., especially using residence times of 0.2 to 10 h, and with an excess of GlcNAc in comparison with Pyr which is subsequently added if necessary. Epimerase and lyase are preferably present in the reactor in a ratio of activities which is equivalent to the reciprocal value of the quotient of the conversion rates.

Enzymatic process for preparing N-acetylneuraminic acid

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Himmelried, Switzerland

Daniel Gygax