Qiagen Gmbh

Daniel Grölz

Ralf Wyrich

Thorsten Voss

Christian Lenz

Andrea Ullius

Uwe OELMÜLLER

The present invention pertains to a method of preparing a frozen biological sample, comprising the steps of fixing the biological sample with a non-crosslinking fixative solution, incubating the fixed biological sample in an aqueous solution comprising a cryoprotectant, and freezing the cryoprotected biological sample. The method advantageously allows to preserve both, morphology of the sample as well as biological components such as nucleic acids and proteins, in high quality for subsequent analysis. The method is robust, simple and neither requires laborious steps associated with paraffin-embedding nor immediate freezing of the sample. Also provided are advantageous uses and kits.

Method of preparing a frozen biological sample

The present invention provides a method for preparing a sterilized composition suitable for stabilizing an extracellular nucleic acid population of a biological sample. Also provided are sterilizable compositions, wherein the compositions in sterilized form are suitable for stabilizing an extracellular nucleic acid population of a biological sample. Further useful methods, devices, kits and uses are also provided. Further sterilisable and sterilized compositions described herein are also suitable to stabilize intracellular nucleic acids (e.g. intracellular DNA such as genomic DNA and/or intracellular RNA) and cell characteristics, such as e.g. cell surface proteins and/or the cell morphology.

Method of preparing sterilized compositions for stabilization of extracellular nucleic acids

A formal-in-free fixative composition, suitable for the fixation of cells in particular in liquid samples, the use of said fixative for the treatment of biological samples, a method for the treatment of cell-comprising liquid samples, a kit comprising said fixative and a method for diagnosis of cell-comprising biological material samples.

Fixative composition for cell-comprising liquid samples and methods and kit thereof

The present invention provides methods, compositions and devices for stabilizing the extracellular nucleic acid population in a cell-containing biological sample using butanamide.

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Hilden, Germany

Daniel Grölz