Genentech, Inc.

Other

Genetech, Inc.

Genencor, Inc.

Daniel G Yansura

Richard L Vandlen

William I Wood

Audrey Goddard

Paul J Godowski

Colin K Watanabe

Ellen Filvaroff

Sherman Fong

Daniel Tumas

Kenneth J Hillan

Jian Chen

P Mickey Williams

Hanzhong Li

Austin L Gurney

James A Ernst

Dorothea E Reilly

Hok Seon Kim

Laura C Simmons

J Christopher Grimaldi

Herbert L Heyneker

Melissa A Starovasnik

Sarah G Hymowitz

Dennis G Kleid

Giuseppe F Miozzari

Menno VanLookeren

James A Wells

James Pan

Scott Douglas Power

Dorothy French

Menno Van Lookeren

Christopher Grimaldi

Robin M Adams

David Brian Powers

Justin M Scheer

Ralph H Schwall

Arthur D Levinson

Dennis J Henner

Kirk James Hayenga

Christoph Spiess

Ronald A Hitzeman

Ernst H Rinderknecht

Eriks Sasha Paegle

Chung-Cheng Liu

James A Wilkins

Tim Breece

Austin L Gumey

Laura Klimowski

Dorothea Reilly

Christopher J Grimaldi

Arthur Jyh-Yen Huang

Menno Van Lockeren

Described herein are methods for the efficient production of antibodies and other multimeric protein complexes (collectively referred to herein as heteromultimeric proteins) capable of specifically binding to more than one target. The targets may be, for example, different epitopes on a single molecule or located on different molecules. The methods combine efficient, high gene expression level, appropriate assembly, and ease of purification for the heteromultimeric proteins. The invention also provides methods of using these heteromultimeric proteins, and compositions, kits and articles of manufacture comprising these antibodies.

Production of heteromultimeric proteins

The present invention is directed to novel polypeptides having sequence identity with IL-17, IL-17 receptors and to nucleic acid molecules encoding those polypeptides. Also provided herein are vectors and host cells comprising those nucleic acid sequences, chimeric polypeptide molecules comprising the polypeptides of the present invention fused to heterologous polypeptide sequences, antibodies which bind to the polypeptides of the present invention and to methods for producing the polypeptides of the present invention. Further provided herein are methods for treating degenerative cartilaginous disorders and other inflammatory diseases.

Nucleic acids encoding receptor for IL-17 homologous polypeptides and therapeutic uses thereof

Methods for producing membrane-spanning polypeptides in high yields, with native conformation, and/or in soluble form include solubilizing in non-ionic or zwitterionic detergents, as well as use of promoters and expression vectors for expressing high yields of membrane-spanning polypeptides in bacterial cells. Mutated promoters provide tight control of membrane-spanning polypeptides in bacterial cell hosts.

Methods for producing soluble membrane-spanning proteins

IL-17 receptor-like polypeptides and therapeutic uses thereof

Pharmaceutical compositions, kits, and therapeutic uses of antagonist antibodies to IL-17E

Methods for producing soluble membrane spanning proteins

The invention provides methods and compositions for improved expression and production of recombinant antibodies in host cell expression systems. In particular, prokaryotic expression and production of antibodies with modified hinge cysteine residues are provided. The invention further provides compositions, kits and articles of manufacture for practicing methods of the present invention.

Methods and compositions for increasing antibody production

Vectors for producing polypeptides heterologous to prokaryotes are described comprising, along with the polypeptide-encoding nucleic acid, anti-termination nucleic acid that inhibits intragenic transcription termination with a non-lambda promoter therefor and/or nucleic acid encoding a GreA or GreB protein and a promoter therefor. Also described are processes for producing a heterologous polypeptide in prokaryotic host cells utilizing such elements to improve the quality and/or quantity of heterologous polypeptide produced.

Process for production of polypeptides

The present invention provides methods and compositions for improved expression and production of recombinant antibodies in prokaryotic expression systems. Particularly contemplated are prokaryotic expression and production of full length aglycosylated antibodies. The antibody products of the invention can be used in various aspects of biological research, diagnosis and medical treatment.

Separate-cistron contructs for secretion of aglycosylated antibodies from prokaryotes

IL-17 related mammalian cytokine polypeptides (IL-17E)

The present invention is directed to novel polypeptides and to nucleic acid molecules encoding those polypeptides. Also provided herein are vectors and host cells comprising those nucleic acid sequences, chimeric polypeptide molecules comprising the polypeptides of the present invention fused to heterologous polypeptide sequences, antibodies which bind to the polypeptides of the present invention and to methods for producing the polypeptides of the present invention.

IL-17 homologous polypeptides and therapeutic uses thereof

Methods for the controllable expression in high yields of heterologous proteins under the control of a modified tryptophan promoter-operator system are disclosed. The system employed involves sequential resort to tryptophan repression and derepression, as well as deletion of an attenuator function native to the tryptophan operon. Means are also disclosed for effecting deletions at any given point within a gene fragment and for the ready identification of transformant bacteria via triple ligation procedures.

Bacterial polypeptide expression employing tryptophan promoter-operator

Growing community of inventors

Pacifica, CA, United States of America

Daniel G Yansura