University of Minnesota

Iowa State University Research Foundation, Inc.

Cellectis

Medicago Inc.

Cellectis S.a.

University of Iowa Research Foundation

Daniel F Voytas

Feng Zhang

Adam J Bogdanove

Luc Mathis

David A Wright

Bruce Robert Blazar

Jakub Tolar

Mark J Osborn

Thomas Stoddard

Marc-Andre D'Aoust

William Haun

Benjamin Clasen

Michael F Maher

Ryan A Nasti

Jin Li

Paul B McCray, Jr

Nicholas J Baltes

Song Luo

Patrick Sinn

Michael Kyba

Rita Perlingeiro

Fabrizio Rinaldi

Sige Zou

Xiaowu Gai

Junbiao Dai

Erin Doyle

Clarice Lauer Schmidt

Tomas Cermak

Li Wang

Michelle Christian

Adam Bogdanove

Materials and methods for inducing genetic alterations in meristematic plant tissue are provided herein.

Delivery of developmental regulators to plants for the induction of meristematic tissue with genetic alterations

Materials and methods are provided for making plants (e.g.,varieties) that are suitable for producing therapeutic polypeptides suitable for administration to humans and animals, particularly by making TAL effector endonuclease-induced mutations in genes encoding xylosyltransferases and fucosyltransferases.

Mutantplant or cell with reduced XylT and FucT

Method for making nicotiana plants with mutations in XylT and FucT alleles using rare-cutting endonucleases

Systems and methods for gene targeting in plants, including systems and methods that include the use of geminiviruses and customizable endonucleases.

Gene targeting in plants using DNA viruses

Materials and methods related to gene targeting (e.g., gene targeting with transcription activator-like effector nucleases; 'TALENS') are provided.

TAL effector-mediated DNA modification

The invention is directed to transcription activator-like effector nuclease (TALEN)-mediated DNA editing of disease-causing mutations in the context of the human genome and human cells to treat patients with compromised genetic disorders.

TALEN-based gene correction

Materials and methods are provided for makingvarieties with decreased accumulation of reducing sugars and acrylamide in cold-stored potatoes, specifically, by making TALE-nuclease-induced mutations in genes encoding vacuolar invertase.

Potatoes with reduced cold-induced sweetening

Materials and methods for making plants (e.g.,varieties) with decreased levels of amylose are provided herein. The methods can include making mutations in the gene encoding granule bound starch synthase (GBSS), where the mutations are induced using a rare-cutting endonuclease.

Potatoes with reduced granule-bound starch synthase

Talen-based gene correction

Materials and methods are provided for making soybean varieties that have altered oil composition as a result of mutations in the FAD2-1A and FAD2-1B genes.

Modifying soybean oil composition through targeted knockout of the FAD2-1A/1B genes

Provided herein is a general method of correcting a gene, for example a gene with many known mutations, such as a gene with mutations in many different exons which could vary from subject to subject (e.g., patient), as well as a set of tools (TALENs and gene targeting vectors) to accomplish such method.

Gene targeting methods and tools

Materials and Methods related to gene targeting (e.g., gene targeting with transcription activator-like effector nucleases; 'TALENS') are provided.

Growing community of inventors

Falcon Heights, MN, United States of America

Daniel F Voytas