Abl IP Holding LLC

Bytelight, Inc.

Agilent Technologies, Inc.

Leland Stanford Junior University

Byelight, Inc.

University and Agilent Technologies, Inc.

Daniel E Ryan

Aaron T Ganick

Emanuel Paul Malandrakis

Kelby Edward Green

Konstantin Klitenik

Peter Staats

Douglas J Dellinger

Rob Sumner

Robert Kaiser

Joel Myerson

Stephen Haight Lydecker

Mitri J Abou-Rizk

Jeffrey Robert Sampson

Matthew Hebden Porteus

Rasmus O Bak

Ayal Hendel

Joe Clark

Robert Sumner, Jr

Gary Fuchs

Andrew Kennedy

Subhadeep Roy

Provided herein are compositions and methods for inducing CRISPR/Cas-based editing of a target nucleic acid (e.g., target DNA or target RNA) in vitro or in a cell, using modified prime editing guide RNAs (pegRNAs) that incorporate one or more chemically-modified nucleotides. The modified pegRNAs disclosed herein may be used to induce Cas-mediated incorporation of one or more nucleotide changes and/or targeted mutagenesis of a target nucleic acid. The nucleotide change can include, e.g., one or more nucleotide changes, an insertion of one or more nucleotides, or a deletion of one or more nucleotides.

Guide RNAs with chemical modification for prime editing

Provided herein are methods for inducing CRISPR/Cas-based gene regulation (e.g., genome editing or gene expression) of a target nucleic acid (e.g., target DNA or target RNA) in a cell. The methods include using modified single guide RNAs (sgRNAs) that enhance gene regulation of the target nucleic acid in a primary cell for use in ex vivo therapy or in a cell in a subject for use in in vivo therapy. Additionally, provided herein are methods for preventing or treating a genetic disease in a subject by administering a sufficient amount of a modified sgRNA to correct a mutation in a target gene associated with the genetic disease.

Compositions comprising chemically modified guide RNAs for CRISPR/Cas-mediated editing of HBB

Chemically modified guide RNAS for CRISPR/Cas-mediated gene regulation

Chemically modified guide RNAs for CRISPR/CAS-mediated gene regulation

The present invention relates to modified guide RNAs and their use in clustered, regularly interspaced, short palindromic repeats (CRISPR)/CRISPR-associated (Cas) systems.

Guide RNA with chemical modifications

The present invention relates to guide RNAs having chemical modifications and their use in CRISPR-Cas systems. The chemically modified guide RNAs have enhanced specificity for target polynucleotide sequences. The present invention also relates to methods of using chemically modified guide RNAs for cleaving or nicking polynucleotides, and for high specificity genome editing.

High specificity genome editing using chemically modified guide RNAs

An example of a technique for maintaining a previously calibrated mobile device positioning system to account for changes in perturbers of the Earth's magnetic field. A mobile device obtains a corrected heading estimate of the mobile device within a space based on a previously recorded heading correction value. The mobile device also calculates a device heading relative to a previously orientated map of the space. The mobile device subsequently updates the heading correction value with any deviation between the corrected heading estimate and the calculated device heading.

System and method for estimating the position and orientation of a mobile communications device in a beacon-based positioning system

In one aspect, the present disclosure relates to a self-identifying optical transmitter for broadcasting a one-way authentication code using light-based communication. The transmitter may include a memory for storing an identifier of the transmitter, a processor for generating a data signal including an identifier of the transmitter, a modulator for receiving the data signal and generating an electrical signal, the modular generating the electrical signal by modulating the data signal. The transmitter may also include a light source for receiving the electrical signal, converting the electrical signal into an optical signal, and continuously broadcasting the optical signal as an optical data transmission stream. The optical data transmission stream may be used to verify that a receiving mobile device is near the transmitter. The transmitter may also include an optical surface for dispersing the optical data transmission stream as the optical data transmission stream is emitted from the transmitter.

Modulating a light source in a light based positioning system with applied DC bias

In one aspect, the present disclosure related to a method for modifying a beacon light source for use in a light-based positioning system. In some embodiments, the method includes selecting a modulation scheme for the light source, determining a duty cycle for the light source based on the modulation scheme, the duty cycle having a proportion of time the light source is in an on state and a corresponding proportion of time the light source is in an off state, the proportion of time the light source is in an off state resulting in reduced luminosity of the light source, and supplying additional power to the light source to compensate for the reduced luminosity of the light source by the duty cycle.

Method and system for modifying a beacon light source for use in a light based positioning system

Systems and methods are provided that disclose providing a positioning service for devices based on light received from one or more light sources. This light based positioning service uses light information transmitted by each light source to determine the position of the device. The positioning information can include three dimension position information in a building that can then be used to deliver services and information to a mobile device. The content delivered to a mobile device can include multimedia, text, audio, and/or pictorial information. The positioning information along with other location or positioning information can be used in providing augmented reality or location aware services. The light sources can be independent beacons that broadcast information in visible light at a rate that is undetectable by the human eye. Content can be retrieved from a server over a communications connection.

Independent beacon based light position system

Self-identifying one-way authentication method using optical signals

In one aspect, the present disclosure relates to a method for configuring one or more imaging sensors of an imaging device to capture digital images for digital pulse recognition demodulation. In some embodiments, the method includes initializing one or more imaging sensors of the imaging device, determining a subset of the one or more imaging sensors to configure, setting a configuration for each of the one or more imaging sensors of the subset by defining a region of interest as a metering area for each of the one or more imaging sensors of the subset and automatically adjusting a setting for each of the one or more imaging sensors of the subset, and adjusting input parameters of a demodulation function based on a device profile of the imaging device. In some embodiments, the adjusted setting is locked to prevent further adjustment of the adjusted setting.

Method and system for configuring an imaging device for the reception of digital pulse recognition information

Growing community of inventors

San Francisco, CA, United States of America

Daniel E Ryan