Rarecyte, Inc.

Daniel E Campton

Joshua Nordberg

Steve Quarre

David Edward Stewart

Ronald C Seubert

Jonathan Lundt

Lance U'Ren

Jennifer Chow

Arturo Bernardo Ramirez

Melinda R Duplessis

Jackie Stilwell

Evan Castiglia

Yao Sun

Lance U'ren

Steven Quarre

Jonathan Erik Lundt

This disclosure relates generally to detection and, in particular, to detecting objects within a sample or fraction thereof.

Analyte detection

This disclosure is directed to an apparatus, system and method for retrieving a target material from a suspension. A system includes a plurality of processing vessels and a collector. The collector funnels portions of the target material from the suspension through a cannula and into the processing vessels. Sequential density fractionation is the division of a sample into fractions or of a fraction of a sample into sub-fractions by a step-wise or sequential process, such that each step or sequence results in the collection or separation of a different fraction or sub-fraction from the preceding and successive steps or sequences. In other words, sequential density fractionation provides individual sub-populations of a population or individual sub-sub-populations of a sub-population of a population through a series of steps.

Apparatus, system, and method for collecting a target material

Kits, compositions, and methods for labeling target materials or target analytes are considered. The composition can include one or more affinity molecules indirectly conjugated to one or more detection moieties. The kit can be used to detect a single biomarker or multiple biomarkers. The kit can be used to perform a single round of labeling or multiple rounds of labeling.

Kits for labeling of biomarkers and methods of using the same

This disclosure is directed to a method and an attachment solution for adhering a cytological or histological sample, such as buffy coat, to a substrate, such as a microscope slide. The attachment solution includes an attachment base, an anti-coagulant, and a nonsteroidal anti-inflammatory drug. The attachment base may be an alcohol, an acid, an oxidizer, an organohalogen, a ketone, or any combination thereof. Once a sample is obtained, the sample may be re-suspended in the attachment solution or the attachment solution may be added to the sample. The sample may then be dispensed onto an analysis platform as one or more droplets and cured.

Solution and method for adhering suspension components to a substrate

This disclosure is directed to an apparatus, system and method for retrieving a target material from a sample. An enrichment agent may be added to a vessel that contains the sample for positive selection, or, in other words, to select or aid in selecting the target material from amongst the remainder of the sample. The enrichment agent may be, for example, immunomagnetic beads, buoyant beads, high-density beads, chemicals to change the density of the target material, or the like.

Apparatus, system and method for collecting a target material

This disclosure is directed to an apparatus and method for spreading a fluid across a substrate. A spreader for spreading a fluid across a substrate includes a wiper and a frame. The wiper and the frame may mate to permit translation of the wiper across, and with respect to, the frame. The wiper spreads the fluid across the substrate and the frame supports the wiper and the substrate. The frame may also include a ramp to cause a portion of the wiper to lift away from the substrate.

Apparatus for spreading a fluid across a substrate and method of using the same

This disclosure is directed to an apparatus, system and method for retrieving a target material from a suspension. A system includes a displacement fluid, a collector, and a primary vessel. In another implementation, the system includes a processing vessel, a displacement fluid, a collector, and a primary vessel.

This disclosure is directed to an apparatus, system and method for retrieving a target material from a sample. A first depletion agent may be added to a vessel that contains the sample to change the density of a non-target material.

This disclosure is directed to an apparatus, system and method for retrieving a target material from a suspension. A system includes a primary vessel, such as a tube, a collector, and a two-part seal including an internal pliant part and an external constricting part. The internal pliant part may be a float and the external constricting part may be a sealing ring. The system may also include a processing vessel, such as an Eppendorf tube, a syringe or a test tube. The collector is sized and shaped to fit into a primary vessel and funnels the target material from the suspension through a cannula and into the processing vessel. In one implementation, the processing vessel includes at least one displacement fluid to be expelled, such that the at least one displacement fluid pushes the target material into the collector.

This disclosure is directed to an apparatus, system and method for retrieving a target material from a sample. A density-altering agent may be added to a vessel that contains the sample to change the density of a non-target material.

Growing community of inventors

Seattle, WA, United States of America

Daniel E Campton