Creatv Microtech, Inc.

University of Colorado

Trustees of the Colorado School of Mines

Km Labs Inc.

Kapteyn-murnane Laboratories, Inc.

Daniel E Adams

Cha-Mei Tang

Henry C Kapteyn

Margaret Mary Murnane

Peixuan Zhu

Olga V Makarova

Charles G Durfee

Platte T Amstutz

David Kleinfeld

Shuhong Li

Michael Tanksalvala

Jeffrey Squier

Dawn Vitek

Robert M Karl

Jeff Squier

John Czerski

Christina Porter

Elisabeth Shanblatt

Christina L Porter

Giulia F Mancini

Dennis Gardner

Matthew D Seaberg

Bosheng Zhang

Charles S Bevis

Seth L Cousin

Methods of using detection of PD-L1 expression by circulating cancer cells in the screening, monitoring, treatment and diagnosis of cancer in subjects are disclosed. The methods are based on assaying one or more of circulating tumor cells (CTCs), epithelial to mesenchymal transition CTCs (EMTCTCs), cancer associated macrophage-like cells (CAMLs), and cancer associated vascular endothelial cells (CAVEs) isolated from a subject having cancer for PD-L1 expression.

Methods of using PD-L1 expression in treatment decisions for cancer therapy

Circulating tumor cells (CTCs) are associated with metastasis of malignant solid tumors in a patient. Presented here is evidence that CTCs exhibit cell cycle phase variability and that there is a strong correlation between the number of CTCs in a mitotic cell cycle phase and the prospects for long term survival of the subject from which the cells were obtained. Also presented herein are methods of determining the mitotic cell cycle phase of CTCs from a patient having cancer and using the information in grading malignant solid tumors and predicting the likelihood of survival of the patient.

Use of circulating tumor cell mitotic index in cancer stratification and diagnostics

Systems and methods of imaging are described. An imaging system includes a light source configured to projecting a beam of light; a first diffraction grating configured to separating wavelengths of the projected beam of light; a first lens configured to focusing the wavelengths of the projected beam of light projecting from separated by the first diffraction grating; a reticle configured to altering each wavelength of light focused by the first lens; a second lens configured to collimating the wavelengths of light projecting from altered by the reticle; a second diffraction grating configured to multiplexing the collimated light projecting from collimated by the lens; and a third lens configured to projecting the multiplexed light onto an object plane, wherein the multiplexed light is used to generate an image of an object in the object plane.

Systems and methods of single-shot spatial frequency modulation imaging

Apparatus and methods for complex imaging reflectometry and refractometry using at least partially coherent light. Quantitative images yield spatially-dependent, local material information about a sample of interest. These images may provide material properties such as chemical composition, the thickness of chemical layers, dopant concentrations, mixing between layers of a sample, reactions at interfaces, etc. An incident beam of VUV wavelength or shorter is scattered off of a sample and imaged at various angles, wavelengths, and/or polarizations. The power of beam is also measured. This data is used to obtain images of a sample's absolute, spatially varying, complex reflectance or transmittance, which is then used to determine spatially-resolved, depth-dependent sample material properties.

Complex spatially-resolved reflectometry/refractometry

A microfilter having a hydrophilic surface and suited for size-based capture and analysis of cells, such as circulating cancer cells, from whole blood and other human fluids is disclosed. The filter material is photo-definable, allowing the formation of precision pores by UV lithography. Exemplary embodiments provide a device that combines a microfilter with 3D nanotopography in culture scaffolds that mimic the 3D in vivo environment to better facilitate growth of captured cells.

Polymer microfilters, devices comprising the same, methods of manufacturing the same, and uses thereof

Apparatus and methods for forming an image of an object which involves focusing partially to fully spatially-coherent radiation onto a sample and collecting the resulting scattered radiation (the 'standard data set') on an array detector. In addition to the standard dataset, an additional measurement or plurality of measurements is made of a relatively-unscattered beam, using the array detector, which comprises the 'modulus enforced probe (MEP) dataset'. This MEP dataset serves as an extra constraint, called the MEP constraint, in the phase retrieval algorithm used to reconstruct the image of the object.

Modulus-enforced probe

A new sensitive cell biomarker of solid tumors is identified in blood. This biomarker can be used to determine presence of solid tumors, rapid determination of treatment response, early detection of cancer, early detection of cancer recurrence, and may be used to determine therapy.

Capture, identification and use of a new biomarker of solid tumors in body fluids

Apparatus and methods of full spatio-temporal characterization of ultrashort pulses from an input pulse-beam source. An interferometer system generates a first, second, third, and fourth replica of the input pulse-beam such that the second replica has a varying delay with respect to the first replica and the fourth replica has a varying delay with respect to the third replica. A reference plane is imaged onto a nonlinear spectral measurement device based upon the first and second replicas, and the reference plane is also imaged onto a wavefront sensitive (WFS) imaging element based on the third and fourth replicas. The signals from the WFS imaging element and the spectral signal are used to compute a pulse temporal spectral profile of the input pulse-beam.

Apparatus and methods for full spatio-temporal characterization of ultrashort light pulses

A new sensitive cell biomarker of solid tumors and viral infection is identified in blood. This biomarker can be used to determine presence of carcinomas, sarcomas, and viruses, rapid determination of treatment response, early detection of cancer, early detection of cancer recurrence, and may be used to determine therapy.

Use of circulating cell biomarkers in the blood for detection and diagnosis of diseases and methods of isolating them

A preservative reagent for urine is disclosed that increases the stability of cells, such as tumor cells, in urine for a period of several weeks. The preservative reagent comprises polyethylene glycol (PEG), ethanol, paraformaldehyde (PFA), and ethylenediaminetetraacetic acid (EDTA), and optionally pH stabilizing reagents.

Urine preservative reagent for microfiltration

A new sensitive cell biomarker of solid tumors is identified in blood. This biomarker can be used to determine presence of carcinomas, rapid determination of treatment response, early detection of cancer, early detection of cancer recurrence, and may be used to determine therapy.

Apparatus and methods for Coherent Diffractive Imaging with multiple, simultaneous, spatially distinct beams chosen and configured to isolate incoherent sums of beam diffraction such that interference between the multiple beams is not present in the data prior to computationally reconstructing the image. This is accomplished through selecting the multiple beams to be non-interfering modes, or through designing the apparatus such that the interference is not recorded, or through processing the collected data to filter the interference before reconstructing the image.

Growing community of inventors

Thornton, CO, United States of America

Daniel E Adams