The Charles Stark Draper Laboratory, Inc.

Pd-1 Acquisition Group, LLC

Massachusetts Institute of Technology

The Charles Draper Laboratory, Inc.

Daniel Doty

Jeffrey T Borenstein

Nathan Francis Moore

Ashley Lynne Beckwith

Vikki A Spaulding

Maria Isabel Chiu

Hareesh Chamarthi

Bin Feng

Sri Sahitya Vadde

Lei Wang

Hans Albert Felix Scheuplein

Sheila Ranganath

Anhco Nguyen

Thomas McQuade

Aleksander Jonca

Najmia Amirina

Jennifer Watkins-Yoon

Luis Fernando Velásquez-García

Joseph L Charest

Mark Joseph Mescher

Louis Kratchman

Brett C Isenberg

James Gillett Truslow

Alla A Gimbel

Jose A Santos

Hesham Azizgolshani

Brian Cain

Luis Fernando Velásquez-Gracía

Vienna Mott

Luis Velásquez-García

Timothy Haggerty

Alla Gimbel

Thomas Mcquade

A microfluidic device for modeling a tumor-immune microenvironment can include a multiwell plate defining a plurality of microenvironment units fluidically coupled with a plurality of wells. Each microenvironment unit of the plurality of microenvironment units can include one or more compartments. Each microenvironment unit can include a trapping feature positioned within the one or more compartments. The trapping feature can be defined by a portion of at least one of a sidewall or a floor of the one or more compartments. The trapping feature can restrict movement of a tissue sample introduced into the one or more compartments and to allow fluid to flow past the tissue sample. The microfluidic device can include a plurality of micropumps each coupled with a respective well and configured to control movement of a respective fluid sample through each respective well.

Multiwell dynamic model for a tumor-immune microenvironment

This disclosure describes techniques for fabricating a high-resolution, non-cytotoxic and transparent microfluidic device. A material can be selected based on having an optical property with a predetermined degree of transparency to provide viewability of a biological sample through the microfluidic device and a level of cytotoxicity within a predetermined threshold to provide viability of the biological sample within the microfluidic device. An additive manufacturing technique can be selected from a plurality of additive manufacturing techniques for fabricating the microfluidic device based on the selected material to provide a resolution of dimensions of one or more channels of the microfluidic device higher than a predetermined resolution threshold.

Systems and methods for fabricating microfluidic devices

This disclosure describes microfluidic tissue biopsy and immune response drug evaluation devices and systems. A microfluidic device can include an inlet channel having a first end configured to receive a fluid sample optionally containing a tissue sample. The microfluidic device can also include a tissue trapping region at the second end of the inlet channel downstream from the first end. The tissue trapping region can include one or more tissue traps configured to catch a tissue sample flowing through the inlet channel such that the fluid sample contacts the tissue trap. The microfluidic device can also include one or more channels providing an outlet.

Microfluidic tissue biopsy and immune response drug evaluation devices and systems

Antibodies that bind to programmed cell death protein 1 (PD-1), compositions comprising such antibodies, and methods of making and using such antibodies are disclosed.

Anti-PD-1 antibodies

Systems and methods for conducting assays on tissue fragment samples including providing a suspension maintaining pump, and a plurality of fluid reservoirs, wherein the fluid reservoirs are configured to hold a volume of fluid. The fluid reservoirs are fluidically coupled to a microfluidic assay chip, wherein the microfluidic assay chip includes a plurality of parallel assay channels, a first inlet port for introduction of a tissue fragment sample into the microfluidic assay ship, and a second inlet port coupled to the fluid reservoir. Each channel of the microfluidic assay chip also includes a retention barrier configured to trap the tissue fragment sample such that the fluid perfuses through the tissue sample, as well as an outlet port fluidically coupled to a waste receptacle.

Microfluidic system for evaluation of chemotherapeutic and immunotherapeutic drugs

Growing community of inventors

Arlington, MA, United States of America

Daniel Doty