Translate Bio, Inc.

Daniel Crawford

Michael W Heartlein

Frank DeRosa

Shrirang Karve

Anusha Dias

Jonathan Abysalh

Frank Derosa

The present invention relates, in part, to methods for large-scale purification of mRNA. The method includes, at least, a step of centrifuging an mRNA suspension in a centrifuge comprising a porous substrate at a speed sufficient to remove process contaminants and to precipitate purified mRNA composition onto the porous substrate.

Methods for purification of messenger RNA

The present invention relates, in part, to methods for large-scale purification of mRNA. The method includes, at least, steps of forming an mRNA slurry, stirring the slurry, and vacuum or pressure filtering the slurry.

A dry granular material transfer system and method for transfer of granular material such as sand in a dry state through a hose using pressurized air. On a base platform, a conveyor subsystem moves granular material placed on a lower portion to a higher portion while separating clumped material and allowing rocks and debris to fall away, spreading the granular material in a thin layer for ejection at the highest level. A containment subsystem receives granular material and contains the downward fall of material. A portioning subsystem has a turntable with blow-through chambers which receive granular material and move into line with an air inflow tube and air-material outflow tube, and the air-material mixture is blown through the air-material outflow tube and through a transfer hose.

Dry granular material transfer

The present invention provides methods for large-scale production of a composition enriched for full-length mRNA molecules using an SP6 RNA polymerase and compositions produced using such methods and uses thereof.

Large-scale synthesis of messenger RNA

The present invention provides, among other things, multimeric coding nucleic acids that exhibit superior stability for in vivo and in vitro use. In some embodiments, a multimeric coding nucleic acid (MCNA) comprises two or more encoding polynucleotides linked via 3' ends such that the multimeric coding nucleic acid compound comprises two or more 5&#x2032; ends.

Growing community of inventors

Cambridge, MA, United States of America

Daniel Crawford