Her Majesty the Queen in Right of Canada, As Represented by the Minister of Agriculture and Agri-food

Daniel Charles William Brown

Brian Miki

Jiro Hattori

Keqiang Wu

Teresa Martin-Heller

Elizabeth Foster

Lining Tian

Peijun Zhang

Thérèse Ouellet

Hélène Labbé

T-DNA tagging with a promoterless &#x3b2;-glucuronidase (GUS) gene generated transgenicplants that expressed GUS activity either only in developing seed coats, or constitutively. Cloning and deletion analysis of the GUS fusion revealed that the promoter responsible for seed coat specificity was located in the plant DNA proximal to the GUS gene. Analysis of the region demonstrated that the seed coat-specificity of GUS expression in this transgenic plant resulted from T-DNA insertion next to a cryptic promoter. This promoter is useful in controlling the expression of genes to the developing seed coat in plant seeds. Similarly, cloning and characterization of the cryptic constitutive promoter revealed the occurrence of several cryptic regulatory regions. These regions include promoter, negative regulatory elements, transcriptional enhancers, core promoter regions, and translational enhancers and other regulatory elements.

Cryptic regulatory elements obtained from plants

Growing community of inventors

Ilderton, Canada

Daniel Charles William Brown