Combichem, Inc.

Deltagen Research Laboratories, L.l.c.

Daniel B Kassel

Robert A Zambias

Tao Wang

Lu Zeng

Single pass methods and systems for measuring the total masses of individual compounds present in fluid samples in real time, and particularly for fluid samples prepared in microtiter plate format are provided. Individual compounds are isolated by fluid separation column ( ). Portions of the individual compounds isolated by column ( ) are analyzed by mass spectrometer ( ), which determines their concentration on the basis of their molecular weights, and an evaporative light scattering detector ( ), (which determines the total masses of each of the isolated compounds passing therethrough). Total collected masses of the individual compounds are calculated by determining the amount and portion of the isolated compounds diverted into evaporative light scattering detector ( ) during a period of fraction collection.

Automated on-line evaporating light scattering detection to quantify isolated fluid sample compounds in microtiter plate format

Single pass methods and systems for measuring the total masses of individual compounds present in fluid samples in real time, and particularly for fluid samples prepared in microtiter plate format are provided. Individual compounds are isolated by fluid separation column (,). Portions of the individual compounds isolated by column (,) are analyzed by mass spectrometer (,), which determines their concentration on the basis of their molecular weights, and an evaporative light scattering detector (,), (which determines the total masses of each of the isolated compounds passing therethrough). Total collected masses of the individual compounds are calculated by determining the amount and portion of the isolated compounds diverted into evaporative light scattering detector (,) during a period of fraction collection.

Single pass methods and systems for measuring the total masses of individual compounds present in fluid samples in real time, and particularly for fluid samples prepared in microtiter plate format are provided. Individual compounds are isolated by fluid separation column (14). Portions of the individual compounds isolated by column (14) are analyzed by mass spectrometer (16), which determines their concentration on the basis of their molecular weights, and an evaporative light scattering detector (24), (which determines the total masses of each of the isolated compounds passing therethrough). Total collected masses of the individual compounds are calculated by determining the amount and portion of the isolated compounds diverted into evaporative light scattering detector (24) during a period of fraction collection.

Automated on-line evaporation light scattering detection to quantify

A method of analyzing each of a plurality of fluid samples (11-16), comprising, simultaneously spraying a plurality fluid samples (11-16) from electrospray needle array (20) towards mass spectrometer (50); positioning blocking device (40) to block all but one (32-34) of fluid samples (31-34) from reaching mass spectrometer (50); moving electrospray needle array (20) and blocking device (40) relative to one another to permit at least two of the plurality of fluid samples (31, 32) to reach mass spectrometer (50) one at a time; and analyzing the mass spectrum of fluid samples (31, 32, 33, 34) sequentially reaching mass spectrometer (50). A system for analyzing the composition of each of a plurality of fluid samples (31-34), comprising, mass spectrometer (50); multi-head electrospray needle array (20) for spraying fluid samples (31-34) towards mass spectrometer (50); and blocking device (40) positioned between electrospray needle array (20) and mass spectrometer (50), blocking device (40) dimensioned to block all but one (32-34) of fluid sample electrosprays (31-34) from reaching mass spectrometer (50).

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Del Mar, CA, United States of America

Daniel B Kassel