Battelle Memorial Institute

Battelle Development Corporation

Daniel B Anderson

Johnway Gao

Brian S Hooker

Richard T Hallen

Todd R Hart

Andrew J Schmidt

Rodney S Skeen

Justin M Billing

Lesley J Snowden-Swan

Michael R Thorson

Ziyu Dai

Gary D Maupin

Lifang Shi

Gerry R Cysewski

A hydrothermal liquefaction (HTL) system can comprise a biomass slurry source, a first pump in fluid communication with the slurry source and configured to pressurize a biomass slurry stream from the slurry source to a first pressure, a first heat exchanger in fluid communication with the first pump and configured to heat a slurry stream received from the first pump to a first temperature, a second pump in fluid communication with the first heat exchanger and configured to pressurize a slurry stream received from the first heat exchanger to a second pressure higher than the first pressure, a second heat exchanger in fluid communication with the second pump and configured to heat a slurry stream received from the second pump to a second temperature higher than the first temperature, and a HTL reactor configured to produce biocrude from a slurry stream received from the second heat exchanger.

Hydrothermal liquefaction system

Liquefaction processes are provided that can include: providing a biomass slurry solution having a temperature of at least 300&#xb0; C. at a pressure of at least 2000 psig; cooling the solution to a temperature of less than 150&#xb0; C.; and depressurizing the solution to release carbon dioxide from the solution and form at least part of a bio-oil foam. Liquefaction processes are also provided that can include: filtering the biomass slurry to remove particulates; and cooling and depressurizing the filtered solution to form the bio-oil foam. Liquefaction systems are provided that can include: a heated biomass slurry reaction zone maintained above 300&#xb0; C. and at least 2000 psig and in continuous fluid communication with a flash cooling/depressurization zone maintained below 150&#xb0; C. and between about 125 psig and about atmospheric pressure. Liquefaction systems are also provided that can include a foam/liquid separation system. Liquefaction process intermediate compositions are provided that can include a bio-oil foam phase separated from an aqueous biomass solids solution.

Liquefaction processes and systems and liquefaction process intermediate compositions

The present invention provides the promoter clone discovery of a glucoamylase gene of a starch utilizing yeast strainThe isolated glucoamylase promoter is an inducible promoter, which can regulate strong gene expression in starch culture medium.

Isolated yeast promoter sequence and a method of regulated heterologous expression

The present invention provides the promoter clone discovery of an alpha-amylase gene of a starch utilizing yeast strain The isolated alpha-amylase promoter is an inducible promoter, which can regulate strong gene expression in starch culture medium.

Method for using a yeast alpha-amylase promoter

Cloning of a yeast alpha-amylase promoter and its regulated heterologous expression

Synergistically functional, yet separable, cis-acting enhancer elements from the rpL34 promoter are disclosed. These enhancer elements of the instant invention may be used in combination with a plurality of promoters to increase gene expression without affecting the intrinsic specificity of the promoters. Also disclosed are methods for using the enhancer elements of the instant invention as well as vectors and transgenic plants comprising the enhancer elements.

Versatile rpL34 promoter elements and use thereof

Porphyridium cruentum is cultured in an enriched seawater medium using a high initial cell concentration until the productivity of polysaccharide production by the alga is maximized. The whole culture is then extracted by making the culture strongly alkaline, and heat treating it. The culture is cooled, acidified and the polysaccharide precipitated by addition of a water-miscible organic solvent such as ethanol. Very high yields of polysaccharide are obtained, in excess of 4.5 grams per liter of culture.

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Pasco, WA, United States of America

Daniel B Anderson