Centre International De Recherches Dermatologiques

Centre International De Recherches Dermatologigues Galderma (cird

L'oreal

Daniel Asselineau

Braham Shroot

Bruno C Bernard

Yves-Michel Darmon

Khalid Bakkar

Marie-Cecile Lenoir

Maris-Cecile Lenoir

The invention concerns a skin substitute composed of a dermis equivalent covered with an epidermis equivalent. The dermis equivalent is a film formed from a type I collagen gel containing fibroblasts. The epidermis equivalent incorporates a basal membrane equivalent, basal layer cells, in a palisade arrangement attached to the membrane equivalent by hemidesmosomes, cells of the suprabasal layers, granular cells, and flat, keratinized cells arranged near the free surface.

Skin substitute

The invention concerns a process for obtaining a skin substitute and the skin substitute itself. According to this process, a dermis substitute is prepared by mixing contractile cells, a nutritive medium, and collagen, in order to form a contracting gel; this dermis substitute is used as a substrate for an epidermis substitute obtained by culturing, using keratinocytes from an animal or human skin sample. According to the invention, the substrate is implanted with at least one section of a hair follicle retaining at least partially its cellular sheath, this section being implanted perpendicularly to the free surface of the substrate.

Process for creating a skin substitute and the resulting skin substitute

Growing community of inventors

Valbonne, France

Daniel Asselineau