Other

Sano, Inc.

Daniel A Kerschensteiner

Paul N Hayre

The present invention is drawn toward a lateral-flow, colorimetric, gelatinase assay including a sample pad, a reagent pad, a membrane, and an absorbent pad, in that order, wherein the reagent pad has therein a dried form of gelatin-coated nanoparticles in a salt and sugar matrix, and wherein the membrane portion has a test stripe and a control stripe, such that the test stripe is a solution of electrolyte evaporated into a layer, and the control stripe is a solution of anti-gelatin antibody evaporated into a layer. The present invention is also drawn toward a method of detecting one or more gelatinases in a sample fluid using the assay, and methods of determining treatment for wound healing, cancer, ocular rosacea, periodontal disease and equine arthritis using the assay.

Colorimetric gelatinase assay

A solid-phase immunoassay support is provided by treating a test tube liner such as a preformed soft gelatin capsule half with a coupling solution comprising a fixative, a bifunctional coupling agent and a bioactive protein which selectively reacts with the substance which is the subject of the immunoassay. The support is particularly applicable to the radioimmunoassay (RIA) technique for determining the presence and concentration of minute amounts of protein antigens in biological fluid samples and can be readily inserted in an appropriately sized test-tube for easy and quick assays.

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Kernersville, NC, United States of America

Daniel A Kerschensteiner