Charles River Laboratories, Inc.

Dana M Nutter

Eric Stimpson

Thomas Preidel

Norman R Wainwright

Al Fuchs

Michael Braun

Jason Tremblay

Luca Nicoli

Elizabeth Mills

Bruce Joni Tomson

Bala S Manian

Bruce J Tomson

The invention relates to a filtration system for use in a method of determining the presence and/or amount of cells, for example, viable cells, in a liquid sample, and to methods of using and manufacturing such a filtration system. The filtration system includes a cup with an upper portion and a ring portion, where the ring portion is separably coupled to the upper portion.

Filtration system and use thereof

Ringed membrane

Filtration system

Depressor

Membrane holder

Blister

Scanning device adapter

Filtration device

Adapter

Base

Base assembly

Cell capture system

The invention provides a method using a hemocyte preparation, for example,amebocyte lysate, for detecting in a single assay the presence of at least one of a Gram negative bacterium, a Gram positive bacterium, and a fungus in a sample of interest. The method exploits the differential reactivity of Gram negative bacteria, Gram positive bacteria, and fungi with the hemocyte preparation to produce measurable changes in a property, for example, an optical property, of the mixture. Because the Gram negative bacteria, Gram positive bacteria and fungi each produce different changes in a given property, for example, an optical property, it is possible to classify the type of microorganism present in the sample of interest.

Method for classifying a microorganism in a biological sample

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Charleston, SC, United States of America

Dana M Nutter