Nanogen, Inc.

Gamida for Life B.v.

Dan Smolko

Jing Cheng

Michael James Heller

Lei Wu

James P O'Connell

Jonathan M Diver

Shila Jalali

David Willoughby

Ed Sheldon

Edward L Sheldon

Jain Krotz

Havens R John

John J Scott

We have performed separation of bacterial and cancer cells from peripheral human blood in microfabricated electronic chips by dielectrophoresis. The isolated cells were examined by staining the nuclei with fluorescent dye followed by laser induced fluorescence imaging. We have also released DNA and RNA from the isolated cells electronically and detected specific marker sequences by DNA amplification followed by electronic hybridization to immobilized capture probes. Efforts towards the construction of a 'laboratory-on-a-chip' system are presented which involves the selection of DNA probes, dyes, reagents and prototyping of the fully integrated portable instrument.

Integrated portable biological detection system

Microreaction molds and methods of molding very thin films onto substrate surfaces are provided. The molds and molding methods allow for consistency and uniformity in the thicknesses of the films that are applied to the substrate surfaces. The molds may be single composite, such as etched silica, or multicomposite, such as quartz/metal. The molds may further comprise an adjustable molding cavity. The molds of this invention are particularly applicable to generating thin polymeric films onto microchip substrates.

Methods for molding and grafting highly uniform polymer layers onto electronic microchips

We have performed separation of bacterial and cancer cells from peripheral human blood in microfabricated electronic chips by dielectrophoresis. The isolated cells were examined by staining the nuclei with fluorescent dye followed by laser induced fluorescence imaging. We have also released DNA and RNA from the isolated cells electronically and detected specific marker sequences by DNA amplification followed by electronic hybridization to immobilized capture probes. Efforts towards the construction of a &ldquo;laboratory-on-a-chip&rdquo; system are presented which involves the selection of DNA probes, dyes, reagents and prototyping of the fully integrated portable instrument.

Growing community of inventors

Jamul, CA, United States of America

Dan Smolko