Bionebraska, Inc.

Dan Dormady

Fred W Wagner

Jay S Stout

Barton Holmquist

Daniel J Strydom

The invention provides a method of producing a polypeptide having a C-terminal &agr;-carboxamide group. It particularly concerns an enzymatic modification of selected substrate polypeptides which result in cleavage of the substrate polypeptide to form a product peptide with a C-terminal arginine residue having an &agr;-carboxamide group (C-terminal &ldquo;Arg-NH &rdquo;). The method includes contacting an aqueous-based solution including (i) ammonia reagent and (ii) the substrate polypeptide with (iii) clostripain.

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Omaha, NE, United States of America

Dan Dormady