Shijiazhuang Yiling Pharmaceutical Co., Ltd.

Dan Bi

Qian Zhao

Chuangfeng Zhang

Shuo Shen

Shuiying Zhang

Feng Wei

Yupeng Chen

Yunbo Sun

The disclosure provides a method for separating eighteen components in a traditional Chinese medicine composition, including: (1) preparing the traditional Chinese medicine composition into a total extract of the traditional Chinese medicine composition, separating by resin through sequentially eluting with water, 10% ethanol and 30% ethanol, and collecting the 30% ethanol eluate to obtain a 30% ethanol extract; (2) adding the 30% ethanol extract to a reverse phase silica gel ODS-AQ-HG, and separating in a medium pressure separation column to obtain differently numbered elution dry pastes; (3) dissolving the differently numbered elution dry paste with 30% methanol as a solvent, and passing the solution through a 0.45 &#x3bc;m microporous membrane, carrying out a primary separation by high performance liquid chromatography and collecting chromatographic peaks with different retention times, and further purifying by high performance liquid chromatography; finally obtaining the components of eighteen components.

Method for separating eighteen components in traditional Chinese medicine composition

A method for determining the content of menthol in a traditional Chinese medicine composition. The traditional Chinese medicine composition consists of the following medicinal materials:, menthol,, rheum,L.,and. In the method for determining the content, the content of the menthol in the composition is determined by gas chromatography to effectively control the content of menthol in the composition, and the method can save energy and reduce the costs for analysis.

Method for determining content of menthol in preparation of traditional Chinese medicine composition

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Hebei, China

Dan Bi