Tokitae LLC

Damian Madan

Bernhard Hans Weigl

Kevin Paul Flood Nichols

Joshua Davis Bishop

Toan Huynh

Benjamin David Grant

Shannon Weise Stone

James Andrew Roecker

Stephen Paul Harston

Daniel Howard Lieberman

Caitlin E Anderson

David Michael Cate

Elizabeth Jane McClure

Anne-Laure M Le Ny

Eric Nalefski

Ted A Baughman

Embodiments disclosed herein include devices, methods, and systems for direct, selective, and sensitive detection of single-stranded and double-stranded target DNA sequences from various sources using a Cas12a protein. When activated by binding a target DNA sequence, the Cas12a cleaves a tether releasing a reporter molecule that may then be detected. In some embodiments, the systems, methods, and devices may include a filter or membrane that may help to separate the tethered and untethered reporter molecules. These devices, systems, and techniques allow a user to rapidly process samples that may contain the target DNA, without needing to amplify the target sequences. These devices and methods may be used to assay a wide variety of samples and target DNA sources, for the presence or absence of target DNA sequences. Compositions and kits, useful in practicing these methods, for example detecting a target DNA in a biological sample, are also described.

Specific detection of deoxyribonucleic acid sequences using novel CRISPR enzyme-mediated detection strategies

Embodiments include assay devices within a single use, disposable cassette for biochemical assays with at least one fluid well and at least one capillary valve. The capillary valve in combination with motion of porous sheets within the assay leads to addition of liquid reagents at different times to the assay although a user adds the liquid reagents to the cassette simultaneously.

Assay structures for multi-step biochemical assays

Designs of improved canisters for animal semen straw storage in Dewars with cryogenic liquid are described. In some embodiments, the canisters include a layer of cryogen-absorbent material and an inner layer of thermally conductive material including apertures oriented and positioned to direct cryogen vapor into the interior of the container.

Containers for liquid nitrogen storage of semen straws

Growing community of inventors

Issaquah, WA, United States of America

Damian Madan