Roche Sequencing Solutions, Inc.

Kapa Biosystems, Inc.

Daleen Badenhorst

Martin Ranik

Jenny A Johnson

Ashley Hayes

Richard Dannebaum

Severine Margeridon

Aruna Arcot

Monica Herrera

Persis Wadia

The invention is a novel method of sequencing nucleic acids involving making and sequencing a library of double stranded target nucleic acids containing a linear partially single-stranded adaptor.

Generation of double-stranded DNA templates for single molecule sequencing

The invention is a novel method of making and using a library such as a sequencing library of single stranded circular nucleic acid templates via splint ligation. In particular, disclosed are methods of making circular target nucleic acid molecules and libraries of such molecules for downstream analysis such as nucleic acid sequencing. The method comprises the steps of adding universal sequences to nucleic acid molecules, rendering single-stranded these nucleic acid molecules with universal sequences on their ends by contacting with a probe complementary to at least a portion of the universal sequences, and allowing the hybridized probe to enable circularization and formation of single-stranded circular (sscDNA) molecules.

Hybridization-extension-ligation strategy for generating circular single-stranded DNA libraries

Growing community of inventors

Wellington, Canada

Daleen Badenhorst