Illumina Corporation

N K Technology Ltd

Omniome, Inc.

Electronic Scripting Products, Inc.

Pacific Biosciences of California, Inc.

Arizona State University

Other

Electronics Scripting Products, Inc.

Buermann, Dale

Kindwall, Alexander P.

Dale Buermann

Wenyi Feng

Jason Bryant

Michael J Mandella

Bryan Crane

Mark T Reed

Matthew William Hage

Alexander Hsiao

Boyan Boyanov

Tarun Kumar Khurana

M Shane Bowen

Stanley S Hong

Hai Quang Tran

David Louis Heiner

Alex Aravanis

Mark Wang

Michal Lebl

Alexander Triener

Behnam Javanmardi

Jason Harris

John A Moon

Arnold Oliphant

Abdul Rahim Forouhi

Mark J Nibbe

Alex Nemiroski

Sebastian Bohm

Robert C Kain

Hector H Gonzalez-Banos

Erik Allegoren

Stewart R Carl

James Michael Osmus

Poorya Sabounchi

Mark R Pratt

Majid Aghababazadeh

Colin Lloyd Barnes

Erik Lewis Williamson

Rebecca McGinley

Erik D Olson

Kamil Salloum

Harold Philip Swerdlow

Drew R Verkade

Eric Hans Vermaas

Patrick Leung

John Bridgham

Michael John Erickstad

Harry Scott Rapoport

Jonathan Posner

Alexander Kindwall

Donald V Rosenberry

Guanghua G Zhang

Michael Aaron Schroeder

Klaus Maisinger

Yoshinao Taketomi

Frederick G Erie

Daniel Tran

Matias Negatu

Andrew James Carson

Bojan Obradovic

Eugene Pogrebinsky

Isabelle Rasolonjatovo

Gary Schroth

Alexander P Kindwall

Maxim Abashin

Chiara Rodighiero

Subra Sankar

Eric Williamson

Saibal Banerjee

Kevin Benson

Sergey Etchin

Johnny Ho

Xavier Lee

Drew Ival Frerichs

Peter Lundberg

Drew Verkade

Ning Sizto

Jonny Ho

Frederick Erie

Matthew Hage

Mark Pratt

Erik Williamson

Rebecca Mcginley

Systems and methods for conducting designated reactions utilizing a base instrument and a removable cartridge. The removable cartridge includes a fluidic network that receives and fluidically directs a biological sample to conduct the designated reactions. The removable cartridge also includes a flow-control valve that is operably coupled to the fluidic network and is movable relative to the fluidic network to control flow of the biological sample therethrough. The removable cartridge is configured to separably engage a base instrument. The base instrument includes a valve actuator that engages the flow-control valve of the removable cartridge. A detection assembly held by at least one of the removable cartridge or the base instrument may be used to detect the designated reactions.

Systems and methods for biochemical analysis including a base instrument and a removable cartridge

Systems and methods for conducting designated reactions that include a fluidic network having a sample channel, a reaction chamber, and a reservoir. The sample channel is in flow communication with a sample port. The system also includes a rotary valve that has a flow channel and is configured to rotate between first and second valve positions. The flow channel fluidically couples the reaction chamber and the sample channel when the rotary valve is in the first valve position and fluidically couples the reservoir and the reaction chamber when the rotary valve is in the second valve position. A pump assembly induces a flow of a biological sample toward the reaction chamber when the rotary valve is in the first valve position and induces a flow of a reaction component from the reservoir toward the reaction chamber when the rotary valve is in the second valve position.

Systems and methods including a rotary valve for at least one of sample preparation or sample analysis

A scanning apparatus that can include (a) a scan actuator including a linear actuator and a mount for a removeable vessel, wherein the mount has a mechanical fastener that is configured to engage a complementary mechanical fastener on the removeable vessel, and wherein the linear actuator is configured to translate the mount while the mechanical fastener engages the complementary mechanical fastener on the removeable vessel; (b) a reference surface; and (c) a preload configured to urge the vessel to contact the reference surface, wherein the linear actuator is configured to slide the vessel along the reference surface while the preload urges the vessel to contact the reference surface.

Scanning apparatus and methods for detecting chemical and biological analytes

An apparatus for evaluating focus, including (a) a stage configured to hold a specimen; and (b) an optical train including a radiation source, calibration optic, objective and detector, the optical train forming a first path wherein radiation from the radiation source is directed to the calibration optic and then a first portion of the radiation is directed to the detector, thereby forming a first image on the detector, wherein a second portion of the radiation follows a second path from the calibration optic then through the objective to the specimen, wherein the optical train forms a third path wherein radiation reflected from the specimen is transmitted through the objective, then to the detector, thereby forming a second image on the detector, and wherein the radiation that forms the first image is astigmatic.

Calibrated focus sensing

An objective lens is used for DNA sequencing. An example system includes a flow cell, the objective lens, and a camera. Light from the flow cell is imaged by the camera through the objective lens. The objective lens can provide a long working distance; a flat field curvature; a high numerical aperture; and/or a wide field of view.

Method and system for biological imaging using a wide field objective lens

A detection apparatus having a read head including a plurality of microfluorometers positioned to simultaneously acquire a plurality of the wide-field images in a common plane; and (b) a translation stage configured to move the read head along a substrate that is in the common plane. The substrate can be a flow cell that is included in a cartridge, the cartridge also including a housing for (i) a sample reservoir; (ii) a fluidic line between the sample reservoir and the flow cell; (iii) several reagent reservoirs in fluid communication with the flow cell, (iv) at least one valve configured to mediate fluid communication between the reservoirs and the flow cell; and (v) at least one pressure source configured to move liquids from the reservoirs to the flow cell. The detection apparatus and cartridge can be used together or independent of each other.

Integrated optoelectronic read head and fluidic cartridge useful for nucleic acid sequencing

An apparatus can include a vessel, a reference surface, a preload, a scan actuator, and a transmitter. The reference surface can form a structural loop with a detector. The preload can be configured to urge the vessel to contact an area on the reference surface. The scan actuator can be configured to slide the vessel along the reference surface in a scan dimension. The transmitter can be configured to direct signal from the vessel to a detector and/or direct energy from an energy source to the vessel, when the vessel is urged by the preload to contact the reference surface.

Scanning apparatus and method useful for detection of chemical and biological analytes

Scanning apparatus and methods useful for detection of chemical and biological analytes

A system and method for imaging biological samples on multiple surfaces of a support structure are disclosed. The support structure may be a flow cell through which a reagent fluid is allowed to flow and interact with the biological samples. Excitation radiation from at least one radiation source may be used to excite the biological samples on multiple surfaces. In this manner, fluorescent emission radiation may be generated from the biological samples and subsequently captured and detected by detection optics and at least one detector. The detected fluorescent emission radiation may then be used to generate image data. This imaging of multiple surfaces may be accomplished either sequentially or simultaneously. In addition, the techniques of the present invention may be used with any type of imaging system. For instance, both epifluorescent and total internal reflection methods may benefit from the techniques of the present invention.

Compensator for multiple surface imaging

Growing community of inventors

San Diego, CA, United States of America

Dale Buermann