Genetics Institute, Inc.

Acton Biotech Consulting

Translational Medicines, Inc.

Dale Anthony Cumming

Ravindra Kumar

Dianne S Sako

Geertruida Machteld Veldman

Glenn R Larsen

Gray D Shaw

Xiao-Jia Chang

Ray Camphausen

The present invention relates to methods for increasing the diversity of monoclonal antibodies produced against an antigen. The methods of the invention utilize immunization of a murine host defective in one or more enzymes involved in a post-translational modification of a polypeptide or a modification of a lipid, wherein said modification is exposed on a cell surface. The invention also relates to monoclonal antibodies produced by these methods and which are not produced when a normal mouse is immunized with the same antigen. The invention further relates to compositions comprising these monoclonal antibodies, as well as to such monoclonal antibodies bound or conjugated to a toxin, a detectable marker or to a solid support.

Methods for increasing the diversity of monoclonal antibodies produced against an antigen

A novel P-selectin ligand glycoprotein is disclosed, comprising the amino acid sequence set forth in SEQ ID NO:2 or by the amino acid sequence set forth in SEQ ID NO:4. DNA sequences encoding the P-selectin ligand protein are also disclosed, along with vectors, host cells, and methods of making the P-selectin ligand protein. Pharmaceutical compositions containing the P-selectin ligand protein and methods of treating inflammatory disease states characterized by P-selectin- and E-selectin-mediated intercellular adhesion are also disclosed.

P-selectin ligand protein

Nucleic acids encoding P-selectin ligand fusion proteins

Fusions proteins comprising P-selectin ligand proteins are disclosed, including fusions with immunoglobulins, BMPs, AGP and IL-11. Polynucleotides encoding such fusions are also disclosed.

Fusions of P-selectin ligand protein and polynucleotides encoding same

Nucleic acid encoding a novel P-selectin ligand protein

Method of inhibiting P-selectin ligand activity

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Acton, MA, United States of America

Dale Anthony Cumming