Monsanto Technology LLC

Dekalb Genetics Corporation

Dale A Voyles

Michael H Luethy

Alan L Kriz

Thomas M Malvar

Rebecca A Kelly

Mark A Dizigan

Kathleen P Malloy

Mark Anthony Dizigan

Michael Hans Luethy

Disclosed herein are assays for detecting the presence of a lysine-increasing transgenic event based on the DNA sequence of the exogenous DNA construct inserted into the maize genome and of genomic sequences flanking the insertion site. Also provided are transgenic plants having a novel exogenous DNA construct that expresses a dihydrodipicolinic acid synthase, the activity of which results in increased lysine in a plant or plant product.

High lysine maize compositions and methods for detection thereof

Methods and compositions for the expression of transgenes in monocot plants including maize are disclosed. In the invention, gene silencing is avoided by use of monocot-homeologous sequences from plants of the genusfor transformation. Included in these transgene sequences arepromoters, enhancers, coding sequences and terminators. Suitable alternatives to maize-derived transgenes are desirable for expression in maize in that homology-based gene silencing can limit or effectively eliminate transgene expression.

Methods and compositions for expression of transgenes in plants

The invention provides isolated gamma coixin promoters useful for the expression of heterologous coding sequences in transgenic plants. Further provided by the invention are transgenic plants comprising such promoters, expression constructs for the introduction of heterologous coding sequences into plants, and methods of using the plants and nucleic acids provided by the invention.

Disclosed herein are novel DNA constructs encoding dihydrodipicolinic acid synthase that are useful for generating transgenic events. Also provided are transgenic plants harboring such DNA constructs, the expression of which results in increased lysine in the plant or plant product. Also provided are maize plants having LY038 Event and progeny thereof having this event. Additionally disclosed are assays for detecting the presence of a lysine-increasing transgenic event based on the DNA sequence of the exogenous DNA construct inserted into the maize genome and of genomic sequences flanking the insertion site.

High lysine maize compositions and event LY038 maize plants

This invention provides an embryo specific promoter, e.g. derived from the 5' regulatory region of an emb5 gene. Compositions comprising this promoter include DNA constructs useful for plant transformation and plants transformed with such DNA constructs. Further provided are methods for the expression of transgenes in plants using the embryo specific promoter sequences. The methods of the invention include the direct creation of transgenic plants with the emb5 promoter by genetic transformation, as well as by plant breeding methods. The sequences of the invention represent a valuable new tool for the creation of transgenic plants, preferably having one or more added beneficial characteristics.

Maize embryo-specific promoter compositions and methods for use thereof

Methods and compositions for the expression of transgenes in monocot plants including maize are disclosed. In the invention, gene silencing is avoided by use of monocot-homeologous sequences from plants of the genus Coix for transformation. Included in these transgene sequences are Coix promoters, enhancers, coding sequences and terminators. Suitable alternatives to maize-derived transgenes are desirable for expression in maize in that homology-based gene silencing can limit or effectively eliminate transgene expression.

Growing community of inventors

Pawcatuck, CT, United States of America

Dale A Voyles