Beijing Institute for Cancer Research

Dajun Deng

Jun Zhang

Yu Sun

Jing Zhou

Yuan Tian

Hua Bai

Zhaojun Liu

Liankun Gu

Baozhen Zhang

Jiyu Li

Provided are a set of primer pair and probe, having the oligonucleotide sequences as shown in SEQ ID NO: 1, SEQ ID NO: 2 and SEQ ID NO: 3, and a set of primer pair and probe of reference sequence, having the oligonucleotide sequences as shown in SEQ ID NO: 4, SEQ ID NO: 5 and SEQ ID NO: 6. Further provided is the use of the set of primer pair and probe for detecting the common deleted region 'chr9:21970277-21985225,hg19', of CDKN2A in the preparation of a kit for quantitatively detecting the deletion of a human CDKN2A gene copy in a DNA sample to be tested. The new method and the specific primer pair and probe can simply, conveniently and specifically detect the deletion of the CDKN2A gene copy in the sample, and have higher sensitivity than conventional detection methods for copy deletion.

Method for quantitatively detecting deletion of human CDKN2A gene copy, primers and use thereof

Provided in the present invention are a method using in vitro measurement of the content of methylation or demethylation of GFRa1 CpG islands to estimate a risk of tumorigenesis and of tumor metastasis, or postoperative life expectancy, and a nucleotide sequence used.

Methods and nucleotide fragments of predicting occurrence, metastasis of cancers and patients' postoperative survival in vitro

The invention provides a primer group for detecting CpG island methylation of p16 gene using methylation specific fluorescence technique. The primer group comprises a pair of oligonucleotide primers and a fluorescence-labeled probe. Said oligonucleotide primer pair has base sequence represented by SEQ ID NO.1 and SEQ ID NO.2. Said fluorescence-labeled probe has base sequence represented by SEQ ID NO.3 or SEQ NO.4.

Primer group for detecting CPG island methylation of P16 gene using methylation-specific fluorescence technique

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Beijing, China

Dajun Deng