Hewlett-Packard Development Company, L.p.

Daixi Xin

Viktor Shkolnikov

Yang Lei

Ken Ward

In example implementations, an apparatus is provided. The apparatus includes a dielectrophoresis (DEP) separator, an electrical field generator, a tracking system, and a controller. The DEP separator is to separate a plurality of different particles. The electrical field generator is coupled to the DEP separator to apply a frequency to the DEP separator. The tracking system is to track a movement of a type of particles in the DEP separator. The controller is in communication with the electrical field generator to control the frequency and the tracking system to track the separation. The controller is to calculate a cross-over frequency from a cross-over frequency distribution for the type of particles based on a frequency sweep performed on the type of particles and the movement of the type of particles that is tracked.

Dielectrophoresis separator cross-over frequency measurement systems

A three-dimensional object modeling method may include applying a nonrotating nonuniform electric field to apply a dielectrophoretic torque to a three-dimensional object to rotate the three-dimensional object, capturing images of the object at different angles during rotation of the object and forming a three-dimensional model of the object based on the captured images.

Nonrotating nonuniform electric field object rotation

An example system includes a well plate having at least one well, an array of electrodes positioned on a surface of a bottom floor of the well, and a controller to direct electrical voltage to the electrodes to generate a non-uniform electrical field. The electrical field is to direct an object in the electrical field to a target position in the well.

Non-uniform electrical field to position objects

A particle monitoring system may include a flow passage, a particle imager to image a targeted particle within the flow passage, electrodes supported proximate the flow passage, a power source connected to the electrodes and a controller to cause the power source to charge to electrodes so as to (1) apply an electric field balanced with respect to gravity so as to hold, levitate and rotate a targeted particle within the flow passage during imaging and (2) release the targeted particle following the imaging.

Rotating levitated particle imaging

An object separator may include a substrate, a fluid channel supported by the substrate, a pair of electrodes along the fluid channel to form a dielectrophoretic force to interact with an object entrained in a fluid, and an inertial pump supported by the substrate and positioned within the fluid channel to move the fluid along the fluid channel.

Object separating

An object separator may include a substrate, a fluid channel supported by the substrate, a pair of electrodes along the fluid channel to form a dielectrophoretic force to interact with an object entrained in a fluid and an inertial pump supported by the substrate to move the fluid along the fluid channel.

The present disclosure is drawn to microfluidic cellular membrane modification devices. In one example, a microfluidic cellular membrane modification device can include a microfluidic channel including a pumping portion and an electric field portion. An electrode pair can be positioned about the electric field portion. A bidirectional pump can be in fluid communication with the microfluidic channel at the pumping portion to move fluid backward and forward through the electric field portion.

Microfluidic cellular membrane modification devices

A three-dimensional volume modeling method may include rotating a three-dimensional biological object having a translucent outer surface to different angular positions, capturing different two-dimensional images of the three-dimensional biological object, each of the different two-dimensional images being at a different angular position, and modeling an exterior of the three-dimensional biological object based upon the different two-dimensional images. The method may further involve identifying a point of an internal structure of the three-dimensional biological object each of the two-dimensional images and modeling the internal structure of the three-dimensional biological object in three-dimensional space relative to the exterior of the three-dimensional biological object by triangulating the point amongst the different two-dimensional images using a three-dimensional volumetric template of the three-dimensional biological object.

Three dimensional volume imaging

An example method includes capturing a plurality of images of a rotating object using an imaging device optically coupled to a microscope. The method removing a first portion of a model of the rotating object based on a first contour of the rotating object in a first image of the plurality of images. The method includes orienting the model based on an amount of rotation of the rotating object between capture of the first image and capture of a second image of the plurality of images. The method also includes removing a second portion of the model of the rotating object based on a second contour of the rotating object in the second image.

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Palo Alto, CA, United States of America

Daixi Xin