Angel Yeast Co., Ltd

Daiwu Liu

Zhijun Li

Xuefeng Yu

Minghua Yu

Juan Yao

Zhihong Li

Jincheng Lei

A method for processing a lignocellulose material, comprises the following steps: (1) crushing and sieving the lignocellulose material, and collecting granules with a particle size of between 0.08 and 0.1 mm; (2) mixing the granules obtained in step (1) with water, and dispersing through a colloid mill to yield a suspension with a particle size of 40-80 &#x3bc;m; (3) homogenizing the suspension obtained in the step (2) under high pressure to have a particle size of between 10 and 40 &#x3bc;m; and (4) buffering the suspension obtained in the step (3) with a buffer solution of sodium acetate and acetic-acid, adding cellulase, &#x3b2;-glucosidase, and xylanase, and performing zymolysis for 36-72 hours.

Method for processing lignocellulose material

The present invention relates to a composite yeast for high concentration alcohol fermentation, which includes thermostable, acid protease, phytase, cellulose, &#x3b2;-glucanase, and pectinase, and is suitable for the high concentration alcohol fermentation for various raw materials. In addition to normal fermentation, the composite yeast of the present invention can degrade the raw materials, increase the nutrient ingredients in the mash, promote the growth of yeast and provide stress tolerance protection.

Composite yeast suitable for high concentration alcohol fermentation

The present invention relates to a composite yeast suitable for high concentration alcohol fermentation from sugar-containing raw materials, characterizing in that the composite yeast comprises any kind of dried yeast selected from Brewers yeastHansen of, grape wine yeastBeijerinek, and nutritious materials which are necessary for yeast growth, the nutritious materials include: the dried yeast 40-70 parts by weight, a nitrogen source 20-40 parts by weight, a phosphorous source 5-10 parts by weight, an other inorganic salt 2.5-5 parts by weight, a trace vitamin 1-2.5 parts by weight and a bacteriostatic 0.5-1.2 parts by weight. The present invention further relates to a method for preparation of the composite yeast suitable for high concentration alcohol fermentation from sugar-containing raw materials. Using the composite yeast of the present invention to proceed with sugar fermentation can increase fermentation alcoholicity, decrease residual sugar content, and allows the final alcoholicity of standard raw material, such as sucrose to attain to 14.5-15.5% v/v, and the amount of the residual reducing sugar in the fermentation mash is 0-0.1 wt %.

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Yichang, China

Daiwu Liu