Chiba University

Daisuke Umeno

Maiko Furubayashi

Norihiko Misawa

Shinichi Takaichi

Kyoichi Saito

Hiroki Fukutomi

Yuki Kimura

Shigeko Kawai

Kyohei Ouchi

[Problem] Provided are a production method for a multi-input/multi-output-type genetic switch or a transcription factor, and a multi-input/multi-output-type genetic switch or a transcription factor. [Solving Means] The inventors of the present invention have completed a production method for a multi-input/multi-output-type genetic switch or a transcription factor, essentially including the steps of 'fusing two or more transcription factor genes to each other' and 'introducing mutations into the fusion-type transcription factor gene,' and have further succeeded in obtaining a multi-input/multi-output-type genetic switch or a transcription factor by the method.

Multi-input/multi-output gene switch, and method for producing same

The present invention provides a method of producing a carotenoid having 50 carbon atoms which comprises culturing, in a medium, a cell transformed with a mutant phytoene desaturase gene and obtaining the carotenoid having 50 carbon atoms from the culture. The mutant phytoene desaturase gene has an introduced mutation to encode a mutant phytoene desaturase having an enhanced activity to desaturate a carotenoid backbone compound of 50 carbon atoms.

Method for producing carotenoids each having 50 carbon atoms

Provided are: a selection method for a gene switch and a gene circuit, including using, as a selector, an expression vector containing at least a gene sequence whose expression is controlled by a transcription regulatory factor to be expressed when a genetic switch and a genetic circuit including the genetic switch operate, and a promoter sequence operably linked to the gene sequence upstream thereof; and an expression vector to be used in the selection method. This enables an effective selection method for a genetic switch and a genetic circuit, the selection method being able to be conducted within a short time period and with high selection efficiency and less leakiness.

Method for rapidly developing gene switches and gene circuits

Growing community of inventors

Chiba, Japan

Daisuke Umeno