Panasonic Corporation

Daisuke Miyoshi

Hidenobu Yaku

The present disclosure provides a measurement method of telomerase activity with no occurrence of false-negative and high quantitative capability. In the measurement method of telomerase activity of the present disclosure, a solution containing telomerase as a sample solution is mixed with a solution containing primer DNA as a substrate for telomerase even as or after a solution containing non-primer DNA not as a substrate for telomerase is mixed therewith. Non-primer DNA can remove an effect of DNase in the sample solution and prevent occurrence of false-negative. Subsequently, the primer DNA is extended with the telomerase and the extended primer DNA is detected, through which telomerase activity is measured.

Method of extending DNA with telomerase and method of measuring telomerase activity

The method of the present disclosure determines whether a target DNA forms a G-quadruplex using a phenomenon in which thioflavin T generates a strong fluorescence when reacted with the G-quadruplex in the presence of potassium ions.

Method for detecting formation of G-quadruplex

A method is provided for specifically detecting a G-quadruplex, and the like. The method is characterized by including the steps of preparing a solution including an anionic planar phthalocyanine and mixing the solution with a sample solution to obtain a liquid mixture. The solution includes an anionic planar phthalocyanine. The method also includes a step of measuring the absorbance at 640 to 740 nm of the obtained liquid mixture.

Method for detecting G-quadruplex, method for detecting G-quadruplex-forming DNA and method for determining telomerase activity

An object of the present invention is to provide a method for inhibiting a DNA extension reaction by telomerase. More specifically, the present invention relates to a method for inhibiting a DNA extension reaction by telomerase, the method being characterized by including the step of adding an anionic phthalocyanine to a solution containing telomerase, a DNA to be a substrate of a telomerase reaction, and dNTPs.

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Hyogo, Japan

Daisuke Miyoshi