Otsuka Pharmaceutical Company, Limited

Daisuke Koga

Kiyonori Katsuragi

Ryuta Ito

Yoko Saijo

Makoto Matsumoto

Hideaki Tanaka

Kenji Ono

Moritoshi Kinoshita

Tetsuya Oda

Chie Kawaguchi

Mamoru Fujiwara

Ryo Higashiyama

Masataka Togashi

The disclosure provides a method comprising (1) conducting a PCR using a nucleic acid sample obtained from the subject as the template, a forward primer having a nucleic acid sequence of a part of exon 1 of the BCR gene, and a reverse primer having a nucleic acid sequence complementary to a part of exons 2 to 11 of the ABL1 gene, in the presence of a modified nucleic acid having a nucleic acid sequence of a part of exons 2 to 14 of the BCR gene or a nucleic acid sequence complementary thereto; and (2) determining that the subject has the minor BCR-ABL1 gene when the nucleic acid amplification is occurred in the PCR.

Method of detecting minor BCR-ABL1 gene

The present invention provides a means for efficiently amplifying the exons of PKD1 and PKD2 genes, and a primer set that can amplify all the exons of PKD1 and PKD2 genes under a single set of PCR conditions.

Primer set and method for amplifying exons of PKD1 gene and PKD2 gene

A method for quantifying the expression level of human WT1 mRNA conveniently, in a short period of time, and with high sensitivity is provided. The method can be used for diagnosing cancer, such as leukemia and solid cancer, or for determining when to perform bone marrow transplantation. The method is for quantifying the expression level of human WT1 mRNA by one-step RT-PCR and comprises simultaneously subjecting the human WT1 mRNA and a housekeeping gene (mRNA) to reverse transcription and extension reactions carried out sequentially in the same vessel.

Quantification method for expression level of WT1 mRNA

The present invention provides a monoclonal antibody that specifically binds to acid-fast bacillary lipoarabinomannan, particularly tubercle bacillary lipoarabinomannan, the antibody being set forth below:

Anti-lipoarabinomannan antibody and immunoassay for acid-fast bacillary infection using the antibody

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Osaka, Japan

Daisuke Koga