Nissan Chemical Corporation

Daisuke Hatanaka

Hisato Hayashi

Masami Kozawa

Tatsuro Kanaki

Ayako Aihara

Masataka Minami

Shinsuke Tadokoro

Keisuke Morodome

Masashi Iwakami

Keiichiro Otsuka

Katsuhiko Kida

Provided is a method for producing a liquid medium composition, including using a junction conduit structurehaving a structure in which a first inlet conduitand a second inlet conduitare joined together to form an outlet conduit, and flowing a first liquidcontaining a particular compound into the first inflow conduitand flowing a second liquidcontaining a linking substance into the second inlet conduit, and allowing the flows of the both liquids to join together to mix the both liquids, thus forming a liquid medium compositioncontaining structures formed by the particular compound linked via the linking substance dispersed therein while being flown through the outlet conduit

Method and device for producing liquid culture medium composition

Cell aggregate capture device

Floating material capture device

The present invention provides a medium composition containing deacylated gellan gum or a salt thereof, and an acidic polysaccharide or a salt thereof capable of maintaining a random coil state in a divalent metal cation medium and cross-linking via a divalent metal ion, and permitting culture of a cell or a tissue in suspension, wherein a concentration of the deacylated gellan gum or a salt thereof in the medium composition is 0.002-0.01 (w/v) %, a concentration of the acidic polysaccharide or a salt thereof is 0.004-0.1 (w/v) %, and a mass ratio of the acidic polysaccharide or a salt thereof to the deacylated gellan gum or a salt thereof is not less than 1. In addition, the present invention provides a method for isolating a cell or tissue from a culture preparation containing the medium composition and cell or tissue, including applying a shear force to the culture preparation.

Culture medium composition for suspension culture allowing easy cell recovery, and cell recovery method

Cells or tissues are preserved in an unfrozen state in the liquid composition containing deacylated gellan gum or a salt thereof, and an acidic polysaccharide or a salt thereof capable of maintaining a random coil state in a divalent metal cation medium and cross-linking via a divalent metal ion. The acidic polysaccharide may be alginic acid. The liquid composition may further contain a metal cation such as calcium ion and the like.

Method for preserving a cell material in an unfrozen state

Growing community of inventors

Funabashi, Japan

Daisuke Hatanaka