Ajinomoto Co., Ltd.

Nec Electronics Corporation

Renesas Electronics Corporation

Daisuke Ejima

Tsutomu Arakawa

Ryosuke Yumioka

Masayo Date

Yuzuru Eto

Hajime Koyama

Haruna Sato

Kunio Ono

Shigekatsu Tsuchiya

Tsuyoshi Kida

Keiichi Yokoyama

Manabu Kitazawa

Yutaka Sato

Yoshiyuki Takahara

Hiroshiro Shibai

Sho Senda

Naoto Koyama

Yoko Kuriyama

Tomomi Yoshida

Kouhei Tsumoto

Michiro Sasaki

Masayo Washitake

Mayumi Watanabe

Yumi Ando

Takayoshi Fujii

Keisuke Kato

Yuichi Nakahara

The present invention provides a method for producing a refolded protein, the method comprising a step for mixing, inside a micro mixer, a buffer and a solubilization solution for a protein that has lost higher order structure or that has become insoluble.

Method for producing refolded protein by using flow microreactor, and protein refolding apparatus

Culture media, which contain albumin carrying a reduced amount of fatty acid, are useful for culturing stem cells.

Medium for culturing stem cells

The present invention provides a method for producing a multimeric protein composed of a monomeric protein, wherein the monomeric protein is obtained by fusing a protein having an immunoglobulin fold structure to a protein that can serve as a subunit structure, the method including the steps of:

Method for preparing polymeric protein composed of monomeric protein produced by fusing protein having immunoglobulin fold structure to protein capable of serving as subunit structure

The invention provides a virus-inactivating composition with pH of 3.8 to 5.5, containing (A) 0.02 to 0.3 M arginine and (B) a component such as 0.01 to 10 mM flavonoid, polyphenol, or ascorbic acid derivative, 0.005 to 5 mass % of an arginine derivative, and 0.1 to 2.5 mass % of an extract solution of natural product.

Virus-inactivating composition containing low-molecular weight compound and arginine

The present invention provides a method for conveniently producing a protein formulation in which viruses are inactivated, without impairing the quality of the obtained protein formulation, characterized by including the step of exposing the protein formulation contaminated with the viruses to a 0.1-2M aqueous solution of arginine, an arginine derivative, or a mixture thereof, the aqueous solution being adjusted to pH 3.5 to 5. The present invention also provides a virus inactivation method characterized by including the step of contacting a virus-containing object with a 0.1-2M aqueous solution of arginine, an arginine derivative, or a mixture thereof, the aqueous solution being adjusted to pH 3.5 to 5.

Method for inactivating viruses with slightly acidic arginine

The present invention provides a method for producing a protein which has a restored native higher-order structure by bringing a protein which has lost its native higher-order structure into contact at pH 6.5 to 9.0 with a 1 to 3% aqueous solution of a specific surfactant, such as lauroylglutamic acid to obtain a solubilized solution of the protein; and then adding the solubilized solution to a buffer with pH 6.5 to 9.0 containing arginine or an arginine derivative at a concentration of 0.1 to 1.2 M to lower the concentration of the specific surfactant, such as lauroylglutamic acid, in the obtained mixture solution down to 0.02 to 0.275%. According to the present invention, it is possible to easily restore the native higher-order structure of a protein while smoothly removing the surfactant from the protein.

Protein refolding method

The present invention provides a method of purifying an antibody by protein A affinity chromatography. More specifically, the present invention provides a technique relating to an elution buffer solution which provides a good antibody recovery rate without denaturation.

Purification method which prevents denaturation of an antibody

A heat treatment jig includes a holding concave portion to hold an object and a void part formed near the holding concave portion and a thermal history variation of the object in a heat treatment process is reduced thereby.

Heat treatment jig and heat treatment jig set

Aiming at adjusting the height of bump electrodes connected to lands on a substrate, a semiconductor devicehas a first interconnect substrateand a second interconnect substrate. On one surface of these substrates, first landsand second landsare provided. The plane geometry of the second landsis a polygon characterized by the inscribed circle thereof having an area smaller than the area of the inscribed circle of the first land.

Substrate and semiconductor device

A resin layer covering a semiconductor chip on a wiring board is composed of a first resin layer and a second resin layer, wherein the first resin layer and the second resin layer differ in their plan view pattern, satisfying a relation of a&#x3c;b, where 'a' is difference in length in the direction from the center of the board towards the edges between the first resin layer and the second resin layer, and 'b' is difference in length in the direction from the center of the board towards the corners between the first resin layer and the second resin layer.

Semiconductor device

A method to more quantitatively recover a peak of a protein and associated aggregates, hydrophobic proteins, or hydrophobic peptides which are typically difficult to recover and detect as a peak, using a commercially available gel filtration chromatography column and a mobile phase is described. More specifically, a compound, such as arginine, which may be added to the mobile phase, thereby eliminating the unnecessary interaction that occurs between the proteins, peptides, or aggregates and the column stationary phase, is described.

Method for improving recovery yield in protein purification with gel filtration chromatography

The present invention relates to a high-yield process for producing high-activity, high-purity transglutaminase by refolding denatured microorganism-derived transglutaminase by reacting denatured transglutaminase in an aqueous medium under acidic conditions and reconstituting a higher-order enzymatically active state in an aqueous medium at a neutral pH.

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Kawasaki, Japan

Daisuke Ejima