Kyoto University

Eisai R&d Management Co., Ltd.

Murata Manufacturing Co., Ltd.

Osaka University

Sumitomo Pharma Co., Ltd.

Racthera Co., Ltd.

Daisuke Doi

Jun Takahashi

Yuichi Ono

Masayo Takahashi

Atsushi Kuwahara

Bumpei Samata

Kenji Yoshida

Kiyotoshi Sekiguchi

Hirotaka Fujii

Koji Kamada

A cell population comprising Corin- and/or Lrtm1-positive cells was produced by the following steps (1) and (2), from which Corin positive and/or Lrtm1 positive cells are collected using a substance that binds to Corin and/or a substance that binds to Lrtm1, and dopaminergic neuron progenitor cells are produced by performing suspension culture of the Corin positive and/or Lrtm1 positive cells in a culture solution containing one or more nutritional factors:

Method for producing dopaminergic neuron progenitor cell

An antenna element includes a body including laminated insulator layers, a first ground conductor, and a radiation conductor overlapping the first ground conductor along a first direction from the first ground conductor. Guard ground conductors are provided in the body along the first direction to surround the radiation conductor. The guard ground conductors include second ground conductors electrically interconnected, and one or more first interlayer connection conductors piercing the insulator layers. The second ground conductors are shaped to not make a circuit of the radiation conductor, and are positioned at multiple positions relative to up-down directions. The second ground conductors each have an overlapping portion that overlaps the second ground conductor positioned adjacent to the overlapping portion in an upward or downward direction, and a non-overlapping portion that does not overlap the second ground conductor positioned adjacent to the non-overlapping portion in the upward or downward direction.

Antenna element

The present invention provides a method for producing dopaminergic neuron progenitor cells from pluripotent stem cells, which method comprises the steps of: (i) performing adherent culture of pluripotent stem cells on an extracellular matrix in a medium containing a reagent(s) selected from the group consisting of BMP inhibitor, TGF&#x3b2; inhibitor, SHH signal-stimulating agent, FGF8, and GSK3&#x3b2; inhibitor; (ii) collecting Corin- and/or Lrtm1-positive cells from the cells obtained in Step (i) using a substance which binds to Corin and/or a substance which binds to Lrtm1; and (iii) performing suspension culture of the cells obtained in Step (ii) in a medium containing a neurotrophic factor.

Method for inducing dopaminergic neuron progenitor cells

The present invention provides a method for selecting dopaminergic neuron progenitor cells, which comprises detecting any one or more of markers selected from the group consisting of CD15 (SSEA-1), CD24, CD46, CD47, CD49b, CD57, CD58, CD59, CD81, CD90, CD98, CD147, CD184, Disalogangliosid GD2, SSEA-4, CD49f, SERINC4, CCR9, PHEX, TMPRSS11E, HTR1E, SLC25A2, Ctxn3, Ccl7, Chrnb4, Chrna3, Kcnv2, Grm2, Syt2, Lim2, Mboat1, St3gal6, Slc39a12, Tacr1, Lrtm1, Dscam and CD201.

Markers for dopaminergic neuron progenitor cells

Growing community of inventors

Kyoto, Japan

Daisuke Doi