Mitsubishi Kasei Corporation

Mitsubishi Chemical Corporation

Daiji Naka

Kazuhiro Nagaike

Kazuhiro Takahashi

Naomi Kitamura

Keiji Miyazawa

Hirohito Tsubouchi

Yoshiko Yoshiyama

Rie Matsui

Yasushi Daikuhara

Toshiya Yamagishi

Kazuhiko Nagaike

Takehisa Ishii

A hybridoma is selected that produces a monoclonal antibody exhibiting high reactivity to soluble hepatocyte growth factor activator inhibitor-1 (soluble HAI-1), the target monoclonal antibody is prepared from culture supernatant of the obtained hybridoma, and by using the antibody soluble HAI-1 is measured.

Antibody to hepatocyte growth factor activator inhibitor-1 and use thereof

An antibody which recognizes an active hepatocyte growth factor activator (HGFA) and does not substantially recognize inactive HGFA is provided. Also disclosed is a monoclonal antibody thereof, and a hybridoma cell line for producing the monoclonal antibody. There is further provided a method for measuring active HGFA using the antibody, and a method for detecting a disease, by detecting or measuring active HGFA using the antibody.

Specific antibody directed to active hepatocyte growth factor activator and method for using the same

A method for inhibiting the degradation of a hepatocyte-growth factor (HGF) which comprises combining at least one polysaccharide or a derivative thereof with a hepatocyte-growth factor (HGF) is disclosed. The polysaccharides include heparin, hyaluronic acid, dextran, dextran sulfate, heparan sulfate, dermatan sulfate, keratan sulfate, chondroitin, and chondroitin sulfate.

Method of inhibiting the degradation of hepatocyte growth factor

Disclosed is a hepatocyte-growth agent which comprises a polysaccharide or a derivative such as heparin, heparan sulfate, chondroitin sulfate and dextran sulfate and a hepatocyte-growth factor (hHGF) obtained by purification of plasma or recombination, by which activity of the hHGF is strengthened, and an hHGF molecule is stabilized.

Hepatocyte-growth agent

According to the present invention, there are provided hepatic parenchymal cell growth factor obtained by recombinant DNA technology, a gene coding for the factor, an expression vector capable of expressing the gene, a cell, in particular animal cell, transformed with the expression vector, and a process for producing the hepatic parenchymal cell growth factor.

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Yokohama, Japan

Daiji Naka