Fujifilm Corporation

Mitsubishi Rayon Company, Limited

Tokyo Medical and Dental University

Dai Ujihara

Naoyuki Nishikawa

Fujio Yu

Tetsuji Nakamura

Toshihide Yoshitani

Junichi Katada

Hiroyuki Chiku

Fumiaki Watanabe

Miki Sakai

Johji Inazawa

Yoshihiro Aburaya

Issei Imoto

Noriyuki Kasagi

Atsuhiko Wada

Junya Yoshida

Hideyuki Kanehara

Natsuki Oyabu

Tomoya Ohara

Fumiaki Mitsubishi Rayon Co Ltd Watanabe

Miki Mitsubishi Rayon Co Ltd Sakai

Tetsuji Mitsubishi Rayon Co Ltd Nakamura

Fujio Mitsubishi Rayon Co Ltd Yu

An immunochromatography including steps of mixing an antigen-containable specimen and modified magnetic particles, which are magnetic particles modified with a substance having a specific affinity to the antigen; collecting the magnetic particles using magnetism; dissociating the modified magnetic particles to obtain an antigen-concentrated solution by mixing the collected magnetic particles with a dissociation solution, an amount of which is smaller than the antigen-containable specimen; obtaining a neutralized antigen-concentrated solution; spreading gold particle composite bodies on an insoluble carrier having a reaction site at which a second binding substance has been immobilized, in a state where the gold particle composite bodies which are composite bodies of an antigen in the neutralized antigen-concentrated solution and modified gold particles which are gold particles modified with a first binding substance are formed; capturing the gold particle composite bodies at the reaction site; and silver-amplifying the gold particle composite body.

Immunochromatography

An object of the present invention is to provide an immunochromatographic kit and a method, which are capable of detectingwith high-sensitivity and specificity. According to the present invention, an immunochromatographic kit for detectingis provided, the kit including: a label substance modified with a first antibody against lipoarabinomannan; a porous carrier having a reaction site holding a second antibody against lipoarabinomannan; a compound containing silver; and a reducing agent reducing silver ions, in which at least one of the first antibody or the second antibody is a monoclonal antibody.

Immunochromatographic kit and method for detecting mycobacterium tuberculosis

An analytical method aided with a nucleic acid microarray, the nucleic acid microarray having a spot (X 1) onto which a first probe nucleic acid is immobilized, the method includes: allowing a labeled sample nucleic acid (A 1) of a sample to be tested to hybridize with the first probe nucleic acid; providing the spot (X 1) with a labeled verification nucleic acid (B) that has a sequence capable of hybridizing with at least a part of the first probe nucleic acid and is labeled with a label different from the labeled sample nucleic acid (A 1), and allowing the labeled verification nucleic acid (B) to hybridize with at least the first probe nucleic acid at all spots; measuring a labeled quantity value (F 1) of the labeled sample nucleic acid (A 1); and measuring a labeled quantity value (Fc 1) of the labeled verification nucleic acid (B).

Method for analysis using nucleic acid microarray

The present invention provides: a protein having an improved nitrile hydratase activity, whereby heat resistance has been improved when compared with a wild-type nitrile hydratase activity, wherein the amino acid sequence of a nitrile hydratase is modified; a gene DNA encoding the above protein; a recombinant vector having the above gene DNA; a transformant or transductant having the above recombinant vector; a nitrile hydratase collected from a culture of the above transformant or transductant, and a production method thereof; and a method for producing an amide compound.

Heat-resistant nitrile hydratase

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Ashigarakami-gun, Japan

Dai Ujihara