Helmholtz Zentrum Munchen Deutsches Forschungszentrum Fur Gesundheit Und Umwelt

Gsf-forschungszentrum Fur Umwelt Und Gesundheit, Gmbh

Gsf Forshungszentrum Fur Umwelt Und Gesundheit Gmbh Ingolstradter

Eximmium Biotechnologies Gmbh

Dagmar Pich

Wolfgang Hammerschmidt

Reinhard Zeidler

Henri-Jacques Delecluse

Aloys Schepers

Sibille Humme

The present invention provides herpesviruses, such as EBV, which lack at least one viral miRNA. Such herpesviruses lacking at least one viral miRNA are advantageously not capable of packaging their genome into the capsid, thereby producing HVLPs, which are substantially free of their herpesvirus genome or the nucleic acid molecule encoding the proteinaceous part of the HVLP and viral miRNA. Such HVLPs may be used as vaccine.

Means and methods for treating herpesvirus infection

The present invention is directed to a conditional gene vector system and to a host cell, which has been transfected with such a vector system. The present invention is further directed to a combined preparation comprising the vector system of the invention and an interfering agent. Furthermore, a pharmaceutical composition and its use in the treatment of hemophilia, diabetes, rheumatoid arthritis, genetic immunodeficiency, and graft versus host disease is provided.

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The present invention relates to a method for helper virus-free packaging of gene vector DNA into the virus particles of a DNA virus as well as to eukaryotic helper cells for helper virus-free packaging of gene vector DNA into the virus particles of a DNA helper virus wherein a DNA virus having a genome &gE;100 kbp is employed (FIG. 1).

Method for helper virus-free packaging of a gene vector DNA

The invention relates to a method for the preparation of DNA virus vectors capable of replication in eukaryotic as well as in prokaryotic cells as well as to DNA virus vectors prepared by this method. Preferably, the method is used to prepare Epstein-Barr virus vectors.

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Munich, Germany

Dagmar Pich