Ge Healthcare Bio-sciences Ab

Ge Healthcare Bioprocess R&d Ab

Dag Lindström

Anders Larsson

Hans Berg

James Van Alstine

Henrik Ihre

Philippe Busson

Anders Hagvall

Eva Holmgren

David Buckley

Joachim Stadler

Jan Bergström

Maria Amersham Biosciences Holm

Eva Amersham Biosciences Holmgren

Anders Amersham Biosciences Hagvall

Hans Amersham Biosciences Berg

Henrik Amersham Biosciences Ihre

Anders Amersham Biosciences Larsson

The present invention relates to a method of isolating at least one plasmid from other components of a liquid, which method comprises the steps of providing a separation matrix comprised of one or more porous carriers, which carrier(s) present anion exchange groups on external surfaces as well as pore surfaces and a pore size distribution that does not allow access of plasmids to pore surfaces; contacting said matrix with the liquid to allow adsorption of the plasmids to ligands present on the separation matrix; contacting an eluent with the separation matrix to release the plasmids and recovering plasmids from a fraction of said eluent. Thus, the present method allows the plasmids to adsorb to the external surfaces of the matrix, while other components such as RNA is adsorbed onto the pore surfaces. In one embodiment, the matrix presents a DNA exclusion limit of at least about 270 base pairs; such as at least about 1,000 base pairs.

Plasmid purification

Growing community of inventors

Vattholma, Sweden

Dag Lindström