University of Georgia Research Foundation, Inc.

Daehwan Chung

Janet Westpheling

Joel Andrew Farkas

Minseok Cha

Jennifer Huddleston

This disclosure describes recombinantmicrobes designed to produce greater amounts of acetate, H, and/or ethanol than a comparable wild type control. this disclosure also describes methods that generally include growing such recombinant microbes under conditions effective for the recombinant microbes to produce acetate, H, and/or ethanol.

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The present invention relates to the discovery of a novel restriction/modification system in. The discovered restriction enzyme is a HaeIII-like restriction enzyme that possesses a thermophilic activity profile. The restriction/modification system also includes a methyltransferase, M.CbeI, that methylates at least one cytosine residue in the CbeI recognition sequence to mC. Thus, the invention provides, in various aspects, isolated CbeI or M.CbeI polypeptides, or biologically active fragments thereof; isolated polynucleotides that encode the CbeI or M.CbeI polypeptides or biologically active fragments thereof, including expression vectors that include such polynucleotide sequences; methods of digesting DNA using a CbeI polypeptide; methods of treating a DNA molecule using a M.CbeI polypeptide; and methods of transforming acell.

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Athens, GA, United States of America

Daehwan Chung