Korea Institute of Science and Technology

Dae-Ro Ahn

Eun Gyeong Yang

Hyung Jun Ahn

Dong Yun Shin

Eun-Gyeong Yang

Dongyun Shin

He-Chul Ahn

Thai Bao Dieu Hien

Hyun-Ju Cho

Ki-Cheol Han

Hee-Chul Ahn

Jong Soo Lim

The present invention relates to three-dimensional self-assembled nucleic acid nanoparticles, a drug delivery system comprising the same, and a pharmaceutical composition for the prevention or treatment of acute kidney injury, which comprises the same. The three-dimensional self-assembled nucleic acid nanoparticles of the present invention, which have a tetrahedral structure, exhibit an excellent renal-targeting ability, and thus the nanoparticles conjugated with the pharmaceutically active ingredient for p53 exhibit excellent p53 and caspase 3 expression reductions in vitro and in vivo, and can thereby be applied to the prevention or treatment of acute kidney injury.

Three-dimensional self-assembled nucleic acid nanoparticles and use thereof

The present invention relates to a drug carrier having L-DNA nanocage structure prepared by using L-DNA, the mirror form of natural DNA, as a backbone. The drug carrier of the present invention has very superior cellular uptake efficiency and serum stability, so that it can be applied to deliver various drugs into cells usefully.

Drug carrier having L-DNA nanocage structure

The present invention relates to a DNA sequencing method using a nucleoside triphosphate with a fluorescent blocking group on its 3'-OH end as a reversible terminator. Further, the present invention relates to sequencing-by-synthesis method using the mono-modified reversible terminator (MRT), the novel nucleotide monomer having a reversible fluorescent blocking group removable chemically or enzymatically at its 3&#x2032;-OH end. The sequencing method of the present invention facilitates sequencing of bases inserted by terminating extension of a nucleotide chain by the nucleotide monomer and then detecting fluorescence signal from 3&#x2032;-OH end. At this time, after analyzing the fluorescence signal, the blocking group conjugated to the 3&#x2032;-OH end can be effectively removed, indicating that a free 3&#x2032;-OH functional group can be successfully restored, so that the next monomer insertion is possible, making continuous sequencing possible.

3'-o-fluorescently modified nucleotides and uses thereof

A method for quantitative analysis of interactions between fluorescein-labeled HIF-1&#x3b1; (alpha) C-terminal peptides and cAMP-responsive element binding protein (CBP) or p300 proteins, and a method of screening inhibitors against the formation of HIF-1&#x3b1;-p300 or HIF-1&#x3b1;-CBP protein complexes using the above method is described.

Method for quantitative analysis of interactions between HIF-1alpha C-terminal peptides and CBP or p300 proteins and method of screening inhibitors using the same

A method and a kit for detecting a target protein in a sample with a signal amplification strategy are provided. The signal amplification strategy is established for the aptamer-based molecular recognition of a target protein with concomitant release of single-stranded DNA (G-DNA), which binds complementarily to a single-stranded RNA comprising a fluorophore and a quencher ('F-RNA-Q'). The fluorescence-quenched RNA is then degraded by RNase H to result in a fluorescence signal, and the undamaged G-DNA is recycled to yield fluorescence amplification.

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Seoul, South Korea

Dae-Ro Ahn