Chonnam National University

Korea Institute of Ocean Science & Technology

Dae Eun Cheong

Geun Joong Kim

Sung Hwan You

Sang-Oh Ahn

Hye Ji Choi

Su Kyoung Yoo

Dong Hyun Lee

Jung Hyun Lee

Chul-Ho Yun

Chul Ho Yun

Geun-Joong Kim

Young Jun An

Kyeong Won Lee

Eun Seo Jo

Su-Kyoung Yoo

Hun-Dong Lee

Hye-Ji Choi

Ho-Dong Lim

Eung Sam Kim

Jeong Sun Kim

Thien Kim Le

Hyung Soon Yim

Jae Hong Park

A method of expressing hydrophobin in a soluble form and a method of purifying hydrophobin, and more particularly, to a method of expressing a recombinant fusion protein including hydrophobin in a soluble form in a host cell and then purifying the expressed recombinant fusion protein are provided.

Method for soluble expression and purification of hydrophobin

An NADPH-regeneration system based on monomeric isocitrate dehydrogenase (IDH) and a use thereof. Specifically, the present invention relates to a recombinant vector including a polynucleotide encoding an isocitrate dehydrogenase recombinant protein derived from(CgIDH) and an isocitrate dehydrogenase recombinant protein derived from(AvIDH), a method for producing the recombinant protein, and an NADPH-regeneration system using the recombinant protein produced by the method. The enzyme in a monomeric form that may be efficiently used in the NADPH-regeneration system in the transformant into which the recombinant vector was introduced, was found, and the NADPH-regeneration system using the enzyme in a monomeric form has a very high utility value as biological parts and biocatalyst materials that provides NADPH to the NADPH-dependent enzyme.

Methods of use for an NADPH-regeneration system based on a monomeric isocitrate dehydrogenase

A recombinant vector according to an embodiment of the present invention may produce fibroblast growth factor 19 (FGF19) having enhanced solubility. The synonymous codon substitution variant fibroblast growth factor 19 (scvhFGF19) and chaperone &#x394;ssDsbC may be simultaneously and independently expressed in a host cell into which the recombinant vector is introduced, thereby it is possible to overexpress the fibroblast growth factor 19 in a soluble state.

Recombinant vector and method for producing recombinant fibroblast growth factor 19 using the same

A recombinant vector according to an embodiment of the present invention may be used for producing a reconstituted cytochrome P450 oxygenase-reductase fusion protein. Oxygenase and reductase may be independently expressed in a host cell into which the recombinant vector is introduced, and then may be fused with a split intein so that it is possible to increase the heme content contained in the active site of the oxygenase and improve enzyme activity and stability.

Recombinant vector and method for producing reconstituted cytochrome P450 oxygenase-reductase fusion protein using the same

Provided is a real-time imaging method of NAPDH-dependent metabolic activity in a cell and a use thereof, and more particularly, an imaging method capable of measuring concentration gradient and change in an intracellular coenzyme using a mutant of metagenome-derived blue fluorescent protein (mBFP) in real-time and an application method thereof. In particular, the method of imaging and quantifying the coenzyme contained in various biological samples may be performed by improving fluorescence in a short time even under anaerobic conditions, without adding a substrate required for coenzyme measurement or conversion without destroying cells, and without consuming the time required for formation of a special structure (fluorophore) for generating fluorescence unlike the existing quantitative systems. In particular, provided is a stable quantitative/imaging technique that is directly applicable to biological samples having various complex compositions without signal interference caused by impurities by removing an inherent enzymatic activity of mBFP.

Real-time imaging method of NAPDH-dependent metabolic activity in cell and use thereof

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Gwangju, South Korea

Dae Eun Cheong