Purdue Research Foundation

Mor-Nuco Enterprises, Inc.

Advanced Research & Technology Institute

Wayne State University

Indiana University

D James Morre

Thomas Hughes Corbett

Frederick A Valeriote

Paul A Grieco

James Jinpal Kim

Brandon Eugene Hostetler

Dorothy M Morré

Charles Moncousin

Guy Auderset

Thomas M Kloppel

Thomas W Keenan

John Orczyk

Cancers of different cellular or tissue origins express different ENOX2 cancer isoforms or combinations of isoforms and shed these proteins into the circulation. Herein are disclosed methods both for cancer detection and diagnosis of particular origin, based on the patterns and molecular weights of the isoforms which allow the identification of the cell type and or tissue of origin of the neoplasm. Relative ENOX2 amounts are proportional to tumor burden and provide a reliable measure of response to therapy and disease progression. Also provided is the amino acid sequence to which the scFv antibodies bind as the molecular basis for the specificity of the test.

Methods and compositions for single chain variable region ENOX2 antibodies for cancer detection and diagnosis

The present invention includes a semi-quantitative method for the detection of ENOX2 transcript variants from one or more anti-ENOX2 antibody-binding spots on a blot comprising the steps of: electrophoretically separating proteins from a concentrated blood, serum, or plasma sample from the subject; transferring the electrophoretically separated proteins to a substrate; separating the one or more ENOX2 transcript variants from the one or more anti-ENOX2 antibody-binding spots; and measuring an ENOX2-catalyzed conversion of an ionic silver or gold to a colloidal silver or gold detected by light scattering from the one or more anti-ENOX2 antibody binding spots on the substrate, wherein each of the one or more spots is indicative of the ENOX2 transcript variant. Also provided is the basis for a point of care test to detect ENOX2 transcript variants based on use of colloidal gold or silver complexes with ENOX2 as a rapid simple test for cancer presence.

Methods and compositions for identification and quantification of ENOX2 transcript variants as indications of cancer presence in blood serum and other body fluids based on gold or silver nanoparticle formation

The present invention includes methods for detecting benign to malignant transformation of a cancer in a subject, comprising the steps of: collecting a sample from the subject prior to electrophoretic protein separation; activating electrophoretically separated ENOX2 transcript variants with an ENOX2 electron donor; and detecting the presence of the one or more activated ENOX2 transcript variants using a pan-ENOX2 detectable binding reagent, wherein the presence of one or more activated ENOX2 transcript variants in the sample is indicative of the malignant transformation of the cancer, whereby a 10 to 100 fold increase in detection sensitivity is obtained for the one or more activated ENOX2 transcript variants when compared to an equivalent non-activated ENOX2 transcript variant.

Methods and compositions for targeted two-dimensional western blot analysis for early cancer detection and cancer diagnosis up to ten years in advance of clinical symptoms of malignant disease

Methods for single chain variable region ENOX2 antibodies for cancer detection and diagnosis

The present invention includes purified and isolated quassinoids and synthetically derived quassinoid analogs based on a picrasane carbon skeleton. Novel sidechains at C-15 incorporating water solubilizing agents such as glycine are discussed. Therapeutic methods taking advantage of anticancer, antiviral, and herbistatic properties of these quassinoids are disclosed, including use against solid tumors and human immunodeficiency virus infected cells.

Therapeutic quassinoid preparations with antineoplastic, antiviral, and herbistatic activity

Therapeutic Quassinoid preparations with antineoplastic, antiviral, and

Thiol compounds are employed in conjunction with compounds which modulate self proliferation, both as to plants and mammals. The thiol compounds find effect in enhancing the effectiveness of auxin like compounds, either as herbicides or in enhancing root formation. In addition, thiol compounds act in conjunction with cytotoxic agents in mammals to allow for lower dosages of the cytotoxic agents while retaining the cytotoxic effect against hyperproliferative diseases, such as cancer. The combination finds particular effect against cells dependent upon an isoform of a surface membrane NADH oxidase for proliferation.

Thiol stimulation of root formation

Therapeutic quassinoid preparations with antineoplastic antiviral, and

Thiol activation of cytotoxic and auxin-herbicidal agents and root

Quassinoid compounds, compositions, and methods of using them for treating cells and solid tumors, and inhibiting plant growth. The quassinoid derivatives herein are 11,20-epoxy-1,11,12-trihydroxypicras-3-ene-2,16 diones: ##STR1##

Therapeutic quassinoid preparations with antineoplastic, antiviral, and

N-Acylated catecholmethylamines are used to inhibit cancer cell proliferation. Specifically, capsaicin is found to inhibit growth at low concentration, but not normal cells.

Capsacinoid compounds as proliferation inhibitors

In humans and experimental animals, serum levels of sialic acid-containing glycolipids are elevated 1.5 to 3 fold. A method is described whereby the ganglioside content is estimated on as little as 0.5 ml of serum or plasma. The procedure permits, based on the analysis of a single biochemical parameter, cancer detection applicable to a wide range of human and animal tumor types.

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West Lafayette, IN, United States of America

D James Morre