Centre National De La Recherche Scientifique

Institut Pasteur

Czeslaw Wychowski

Jean Dubuisson

Gilles Duverlie

Marc Girard

Annette Martin

André Pillez

The invention concerns the use of cells capable of carrying out a process of prenylation of proteins coded by the hepatitis C virus (HCV) genome, such as prenylation of the NS5A protein, for replicating and, if required, the production of HCV or derivative viable mutants, in a suitable culture medium.

Nucleotide sequences coding for the non-structural proteins of the hepatitis C virus

Method for replicating the hepatitis C virus

The invention relates to an active principle of a vaccine, in which the characteristic epitope is incorporated in the capsid of a picornavirus, in particular of poliovirus, used as a vector. This active principle consists of a viable hybrid picornavirus containing an epitope characteristic of a vaccinating protein, and heterologous with respect to the proteins of this picornavirus, in place of one of its own immunogenic epitopes.

Plasmid vectors encoding a protein of a picornavirus

Growing community of inventors

Meurchin, France

Czeslaw Wychowski