Corning Glass Works

Cyrus A Lepp

Robert D Mason

Barry I Bluestein

Gerald Odstrchel

William S Ramsey

Creatine kinase isoenzymes in a liquid sample are determined by means of immobilized antibody-isoenzyme complexes in which measureable isoenzyme activity is retained.

Determining creatine kinase isoenzmes via immobilized antibody-isoenzyme

The common hemoglobin variants A, A.sub.2, F, S, and C are discretely separated by electrochromatography under acidic conditions on an agar gel which utilizes an agar gel having a wet thickness of from about 0.1 to about 0.5 mm. and a potential of from about 60 to about 110 volts. The gel buffer is a citrate buffer having a citrate concentration of from about 0.04 to about 0.065 M and a pH of from about 6.0 to about 6.5. The well buffer is a citrate buffer having a citrate concentration of from about 0.055 to about 0.065 M and a pH of from about 6.0 to about 6.5.

Acidic agar gel electrochromatography of hemoglobins

The common hemoglobin variants A, A.sub.2, F, S, and C are discretely separated by alkaline agarose gel electrophoresis employing (1) an agarose gel having a wet thickness of from about 0.1 to about 0.5 mm., (2) a gel buffer comprising tris(hydroxymethyl)aminomethane, ethylenediaminetetraacetic acid, and boric acid, and having an ionic strength of from about 0.065 to about 0.1 and a pH of from about 8.7 to about 9.1, (3) a well buffer comprising diethylbarbiturate and ethylenediaminetetraacetate and having an ionic strength of from about 0.01 to about 0.11 and a pH of from about 8.0 to about 9.5, and (4) a potential of from about 150 to about 300 volts.

Alkaline agarosse gel electrophoresis of hemoglobins

Solidified, polar, micorbial growth medium containing 8-anilino-1-naphthalenesulfonic acid or a salt thereof. Microbial colonies grown on the medium can be detected fluorimetrically.

Growing community of inventors

Corning, NY, United States of America

Cyrus A Lepp