University of Michigan

Other

Wayne State University

Cynthia Marcelo

Belinda Adamson

Riley S Rees

Cynthia Zuccaro

Thomas E Taddonio

Lenore Rhodes

William J Lindblad

Beverly Marchant

Robert Gilmont

Warren Garner

Stephen E Feinberg

Mary-Ann Mycek

Blake J Roessler

William Lloyd

Leng-Chun Chen

Yasushi Fujimori

Shiuhyang Kuo

Hyungjin Myra Kim

Kenji Izumi

This disclosure provides a method of determining the viability of at least one cell via quantification of nicotinamide adenine dinucleotide (phosphate) (NAD(P)H), flavin adenine dinucleotide (FAD), and/or collagen. The method includes the steps of contacting the at least one cell with light having a wavelength of from 700 to 900 nm, using two photon excitation, or from 335 to 400 nm, using one photon excitation, to induce an optical response from the NAD(P)H, FAD, and/or collagen and measuring the optical response. The method also includes the step of quantifying one or more of an amount, spatial localization, and/or time-dependent response of the NAD(P)H, FAD, and/or collagen utilizing the optical response.

Method of determining the viability of at least one cell

In accordance with some preferred embodiments, without limitation, the present invention comprises compositions, methods and systems for preparation of stem-cell enriched cell populations from sources of biological materials by sorting cell types in relation to size.

Compositions, methods and systems for preparation of a stem cell-enriched cell population

The present invention discloses a composite made of a layer of epithelial cells cultured together with a dermal matrix in a culture medium, which is free of BPE. The dermal matrix is a substantially non-immunogenic human dermis. Also disclosed is an enhanced composite where a biological agent has been introduced into the epithelial cells. The invention also comprises methods of making and implanting both the composite and the enhanced composite in an animal. A method of treating disease or injury through the use of the disclosed composites is also part of the present invention.

Mucosal cell composites and methods

Devices and methods for enhancing the healing of wounds, especially chronic wounds (e.g., diabetic wounds), involving the use of keratinocytes are described. Keratinocytes are grown on a transplantable solid support (e.g., collagen-coated beads), and the keratinocyte-coated solid support is placed in an enclosure. The enclosure, in turn, is placed in the wound for use as an interactive wound healing promoter.

Manufacturing therapeutic enclosures

Therapeutic enclosures

Methods and compositions for the treatment of wounds

System for the treatment of wounds

Devices and methods for enhancing the healing of wounds, especially chronic wounds (e.g., diabetic wounds), are provided involving the use of keratinocytes. Keratinocytes are grown on a transplantable solid support (e.g., collagen-coated beads), and the keratinocyte-coated solid support is placed in an enclosure. The enclosure, in turn, is placed in the wound for use as an interactive wound healing promoter. After the enclosure is placed in a wound, the wound may be covered with a dressing. The enclosure is degradable or non-degradable, and is constructed from a membrane or a porous polyester mesh material having pores that are either too small or large enough for keratinocytes to cross. A means may be attached to the enclosure to enable removing the enclosure from a wound.

Wound treatment with keratinocytes on a solid support enclosed in a

Growing community of inventors

Ann Arbor, MI, United States of America

Cynthia Marcelo