Ginkgo Bioworks, Inc.

Massachusetts Institute of Technology

Curt R Fischer

Reshma P Shetty

Austin J Che

Jason R Kelly

Gregory N Stephanopoulos

Hal S Alper

Elke Nevoigt

Andrew A Peterson

The present disclosure identifies pathways, mechanisms, systems and methods to confer chemoautotrophic production of carbon-based products of interest, such as sugars, alcohols, chemicals, amino acids, polymers, fatty acids and their derivatives, hydrocarbons, isoprenoids, and intermediates thereof, in organisms such that these organisms efficiently convert inorganic carbon to organic carbon-based products of interest using inorganic energy, such as formate, and in particular the use of organisms for the commercial production of various carbon-based products of interest.

Chemoautotrophic cells comprising an engineered carbon fixation pathway

The present disclosure identifies pathways, mechanisms, systems and methods to confer production of carbon-based products of interest, such as sugars, alcohols, chemicals, amino acids, polymers, fatty acids and their derivatives, hydrocarbons, isoprenoids, and intermediates thereof, in engineered and/or evolved methylotrophs such that these organisms efficiently convert C1 compounds, such as formate, formic acid, formaldehyde or methanol, to organic carbon-based products of interest, and in particular the use of organisms for the commercial production of various carbon-based products of interest.

Methods and systems for methylotrophic production of organic compounds

Methods for making chemoautotrophic cells by engineering an energy conversion pathway and a carbon fixation pathway

Methods and systems for chemoautotrophic production of organic compounds

A process for the conversion of sugars and/or other biomass to produce hydrocarbons, hydrogen, and/or other related compounds is described. In one aspect, the process includes fermenting biomass to produce one or more organic intermediates, for example, a carboxylic acid, and optionally, hydrogen. The carboxylic acids may then be decarboxylated to produce carbon dioxide and one or more hydrocarbon compounds. Also described are steps to further react the hydrocarbon compounds to form polymers, hydrocarbon compounds having at least 4 carbon atoms, or the like.

Conversion of natural products including cellulose to hydrocarbons, hydrogen and/or other related compounds

The present invention relates to expression cassettes libraries of expression vectors comprising the same, wherein each vector comprises at least one gene of interest and a promoter operatively linked thereto wherein each promoter comprises a nucleic acid, whose sequence is randomly mutated with respect to that of another in the library and cells comprising the same. Methods utilizing either the libraries or cells of this invention, in optimizing gene expression, protein expression, or optimized gene or protein delivery are described.

Promoter engineering and genetic control

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Cambridge, MA, United States of America

Curt R Fischer