Chinese Academy of Sciences

Cui Lv

Jinyong Wang

Mengyun Zhang

Ying Chu Wang

Juan Du

Ying Wang

Lijuan Liu

Yong Dong

Xiaofei Liu

Fangxiao Hu

Qianhao Zhao

Rongqun Guo

Dan Yang

The present invention provides a pluripotent stem cell comprising a co-expression vector in which Runx1 and Hoxa9 are of in tandem, and a T cell differentiated therefrom and application thereof. In the present invention, Pluripotent stem cells inducibly co-expressing exogenous Runx1 and Hoxa9 are successfully established by introducing an exogenous vector co-expressing Runx1 and Hoxa9 into pluripotent stem cells. The pluripotent stem cells are directionally differentiated into T-lineage progenitor cells and will be developed into T cells. The pluripotent stem cell-derived T cells obtained by the method of the present invention are not only functionally normal but also have no tumorigenic risk.

Pluripotent stem cell and T cell differentiated therefrom and application thereof

The present invention relates to a method for inducing the transdifferentiation of B lymphoid cells into T lymphoid cells using transcription factor Hoxb5, and related products and applications thereof. The method of the present invention specifically comprises: introducing Hoxb5, a nucleic acid molecule encoding Hoxb5 or a construct comprising the nucleic acid molecule into the B lymphoid cells to obtain the B lymphoid cells with overexpressed Hoxb5; then implanting the obtained B lymphoid cells into the body of a subject to obtain regenerated T cell progenitor cells by way of transdifferentiation, and then the T cell progenitor cells differentiate into mature T cells with functions. The regenerated T cells obtained using the method of the present invention are not only functionally normal, but also show no risk of tumorigenesis or extremely low risk of tumorigenesis.

Method for regenerating T cells and applications thereof

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Guangdong, China

Cui Lv