Dow Agrosciences LLC

Cristie Min Dewes

Lakshmi Sastry-Dent

Jayakumar Pon Samuel

Zehui Cao

W Michael Ainley

Liu Shen

Aaron Todd Woosley

Ignacio Mario Larrinua

Holly Jean Butler

Vipula Shukla

Avutu Sambi Reddy

James Patrick Connell

Pamela Rene Haygood

Zihua Hu

Anthony Trieu

The present disclosure provides a system and methods for detecting and identifying plant events that contain donor sequences inserted precisely into a targeted genomic loci, and plants and plant cells comprising such targeted genomic loci. The method comprises the steps of amplifying a genomic DNA with a first round of PCR to produce an amplicon from donor sequences inserted in the reverse orientation, wherein the production of the amplicon indicates the presence of the site specific integration event.

Rapid assay for identifying transformants having donor insertion

The present disclosure provides methods for detecting and identifying plant events that contain donor sequences inserted precisely into targeted genomic loci, and plants and plant cells comprising such targeted genomic loci. The method comprises the steps of amplifying a genomic DNA with a first round of PCR to produce a first amplicon from donor sequences inserted in the reverse orientation, amplifying the first amplicon with a second round of PCR and detecting the presence of the second amplicon, wherein the production of the first and second amplicon indicates the presence of the site specific integration event.

Rapid assay for identifying transformants having targeted donor insertion

This invention encompasses the identification and isolation of genes that confer disease control properties in plants, as well as plants comprising such genes. These genes are derived from the following sources:(var. Indica IR7), Papaver rhoeas,and(Rifai 1295-22). The control conferred is against the one or more of the following phytopathogens:, Cercospora zeae-maydis,, and. Further, this invention encompasses other homologous and heterologous sequences with a high degree of functional similarity.

Nucleic acid compositions conferring disease resistance

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Indianapolis, IN, United States of America

Cristie Min Dewes