Danisco US Inc.

Genencor International, Inc.

Genecor International, Inc.

Craig E Pilgrim

Nigel S Dunn-Coleman

Piet Van Solingen

Paulien Neefe-Kruithof

Donald E Ward

Michael C Ward

Kathleen A Clarkson

Suzanne E Lantz

Jayarama K Shetty

Gang Duan

Oreste J Lantero

Michael D Ward

Gopal K Chotani

Donald E Ward, Ii

A dry solids staging fermentation process for producing an end-product, such as ethanol is disclosed said process including an initial fermentation step including combining a first fermentable substrate with one or more starch hydrolyzing enzymes and fermenting organisms in a fermentation vessel and a loading step which includes adding a second fermentable substrate to the fermentation vessel wherein the percent dry solids (% DS) of the fermentation broth increases over time.

Dry solids staging fermentation process

The present invention is related to glucoamylases having at least 80% sequence identity to aglucoamylase having the sequence of SEQ ID NO: 4 and biologically functional fragments thereof. The invention is also related to DNA sequences coding for the glucoamylases, vectors and host cells incorporating the DNA sequences, enzyme compositions and methods of using the glucoamylases in various applications.

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The present invention is directed to novel acid proteases and more specifically to NSP24 family proteases and NSP25 family proteases including biologically active fragments thereof and to nucleic acid molecules encoding said proteases. Also provided are vectors and host cells including nucleic acid sequences coding for the proteases, methods for producing the proteases, enzyme compositions and methods employing said proteases.

Acid fungal proteases

The invention is directed to methods of producing ethanol and decreasing residual starch production in a no cook fermentation comprising contacting granular starch containing substrates with a granular starch hydrolyzing enzyme, a protease, and a fermenting microorganism under suitable fermentation conditions at a temperature below the starch gelatinization temperature of the starch substrate to produce ethanol, wherein the ethanol production is increased and the amount of residual starch is decreased compared to a substantially similar method conducted without the protease.

Acid fungal protease in fermentation of insoluble starch substrates

The present invention is related to glucoamylases having at least 80% sequence identity to a Trichoderma glucoamylase having the sequence of SEQ ID NO: 4 and biologically functional fragments thereof. The invention is also related to DNA sequences coding for the glucoamylases, vectors and host cells incorporating the DNA sequences, enzyme compositions and methods of using the glucoamylases in various applications.

Trichoderma reesei glucoamylase and homologs thereof

The present invention is related to glucoamylases having at least 80% sequence identity to ahaving the sequence of SEQ ID NO: 4 and biologically functional fragments thereof. The invention is also related to DNA sequences coding for the glucoamylases, vectors and host cells incorporating the DNA sequences, enzyme compositions and methods of using the glucoamylases in various applications.

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Beloit, WI, United States of America

Craig E Pilgrim