Becton Dickinson and Company Limited

Cellular Research, Inc.

Grail, Inc.

Craig Betts

Glenn K Fu

Eleen Shum

Christina Fan

Byoungsok Jung

Gretchen Yinbon Lam

This disclosure provides methods and compositions for removing one or more high abundance species from a plurality of nucleic acid molecules. In some embodiments, the methods and compositions can be used for normalizing nucleic acid libraries. In some embodiments, molecular labels are used in conjunction with the methods and compositions disclosed herein to improve sequencing efficiency.

Normalization of nucleic acid libraries

The disclosure provides for methods, compositions, systems, devices, and kits for whole transcriptome amplification using stochastic barcodes.

Methods and compositions for whole transcriptome amplification

The disclosure provides for methods, compositions, and kits for normalizing nucleic acid libraries, for example sequencing libraries.

Methods and compositions for library normalization

Methods for preparing a sequencing library from a DNA-containing test sample are provided. In some embodiments, the methods involve rescuing a partially ligated DNA fragment to enhance library preparation conversion efficiencies. In some embodiments, the methods involve improving recovery of duplex sequence information from double-stranded DNA.

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Menlo Park, CA, United States of America

Craig Betts